Vol 20, No 3 (2017)
- Year: 2017
- Articles: 96
- URL: https://journals.rcsi.science/1028-7221/issue/view/6240
Articles
357-358
THE FUNCTIONAL STATE OF THE IMMUNE SYSTEM IN IRRADIATED PERSONS WITH INCREASED LEVELS OF CHROMOSOMAL ABERRATIONS
Abstract
The decrease of the relative content of CD3+ and absolute content of CD4+ lymphocytes were observed among the personsexposed to the chronic irradiation with elevated levels of chromosomal aberrations in peripheral blood lymphocytes.
Russian Journal of Immunology. 2017;20(3):359-360
359-360
POPULATION NK-CELLS IN PERIPHERAL BLOOD REFLECTS DELINEATED SPECIFIC PROCESSES IN PULMONARY TUBERCULOSIS
Abstract
The purpose of the study was to examine the difference in the number of NK-cells in various forms of pulmonary tuberculosis. During the examined 124 people, of which 99 - have had a form of pulmonary tuberculosis, 25 - were practically healthy people. The study was performed by flow cytometry Coulter Epics XL (BeckmanCoulter, USA). Statistical analysis was performed with the use of programs Statistica for Windows v.6.1. It has been established that the presence of lung tuberculoma is associated with a decrease in the number of NK-cells compared to healthy people by 20 %. Reduced tuberculoma activity is accompanied by lowering of the agreed amount NK-cells by an average factor of two.
Russian Journal of Immunology. 2017;20(3):361-363
361-363
IMMUNOLOGICAL REACTIONS IN PATIENTS WITH LUNG TUBERCULOMA IN DIFFERENT PHASES ACTIVITY
Abstract
The purpose of the study is the definition of characteristics of the response of the immune system of patients with lung tuberculoma in the active phase and phase subsides / moderate activity for the establishment of the laboratory criteria for preoperative evaluation of educational activity. The study was performed with the participation of 160 people, We studied the absorption and functional metabolic activity of neutrophils and monocytes, population composition of cells, expression of adhesion molecules and markers of cell activity, the concentration of cytokines. Determine CD11b expression tests and HLA-DR on monocytes, CD11b - on neutrophils, evaluations populations CD3+HLA-DR+ and CD3+CD4+CD25high can be recommended for preoperative assessment of activity tuberculoma lung.
Russian Journal of Immunology. 2017;20(3):363-365
363-365
FUNCTIONAL FEATURES OF NEUTROPHILIC GRANULOCYTES IN NAZAL WASHES OF RINOCHIRURGICAL PATIENTS IN THE EARLY POST-OPERATING PERIOD
Abstract
The state of neutrophilic granulocytes of nasal flushing in the early postoperative period was analyzed. In the course of the study, it was found that patients in the early postoperative period increased the content of neutrophilic granulocytes in nasal washings, increased the NBT-test of neutrophilic granulocytes, decreased activity, the intensity of the phagocytic reaction of neutrophilic granulocytes, the phagocytic number decreased.
Russian Journal of Immunology. 2017;20(3):365-367
365-367
CLINICAL ROLE OF LOCAL ANTIMICROBIAL FACTORS AND CYTOKINES IN CHILDREN WITH REACTIVE ARTHRITIS
Abstract
Significant local antimicrobial factors of the intestinal biotope and cytokines associated with the variant of the course of reactive arthritis (ReA): IL-17, IL-6, INF-γ, lysozyme, CRB have been determined, which allows them to be used as markers for predicting the course and the outcome of ReA.
Russian Journal of Immunology. 2017;20(3):367-369
367-369
METHODOLOGICAL APPROACHES TO IDENTIFICATION OF PECULIARITIES OF GENETIC POLYMORPHISM AS MARKERS OF EARLY VIOLATIONS OF IMMUNE REGULATION IN CONDITIONS OF CHRONIC EXPOSITION TO CHEMICAL FACTORS
Abstract
The use of targeted gene sequencing (Roche 454 sequencer) in the patients-representatives of European ethnicity with an excessive amount of strontium in the blood made it possible to detect the presence of a significant number of point replacements in 10 genes: MTHFR - gene of methylenetetrahydrofolate reductase (23 replacements); SULT1A1 - sulfransferase gene (41 replacement); SIRT3 - sirtuin gene (13 replacements); NOS 3 - gene of endothelial NO- synthase (19 replacements), and PPARD - receptor gene (6 replacements), activated by peroxisome proliferator: TERT - telomerase gene (14 replacements); FAS -gene of the death receptor (9 replacements); FOXP3 - transcriptional factor of cellular suppression (4 replacements); TP53 - transcriptional factor of onco suppression (7 replacements); VEGF - gene of vascular endothelial growth factor (8 replacements). Sites of these genes can be proposed as markers to manifest the harm to health caused by chemical factors.
Russian Journal of Immunology. 2017;20(3):369-371
369-371
372-374
BACTERIA AS PRODUCERS OF CYTOKINE-LIKE SUBSTANCES
Abstract
In experiments in vitro on the 61 clinical isolates S. aureus (n=23), S. haemolyticus (n=10), Enterococcus faecalis and E. faecalis (n=8), Pseudomonas aeruginosa (n=8), enterobacteria - Klebsiella pneumoniae and E. coli (n=12) using a test-system Multiplex (Luminex, USA) to determine the 15 cytokines (G-CSF, GM-CSF, INF-γ, IL-10, IL-12p70, IL-13, IL-17A, IL-φ, IL-2, IL-4, IL-5, IL-6, MCP-1, MIP-φ, TGF-α) studied the production of cytokine-like substances (CLS). Identified and characterized the intergeneric and intraspecific variability of bacteria in their ability to produce CLS in the environment of cultivation. It is established that isolates of S. aureus are the most active producers of CLS. the possible pathogenetic significance of products CLS bacteria and ways to further study this phenomenon were discussed.
Russian Journal of Immunology. 2017;20(3):374-376
374-376
RESEARCH OF THE SPECTRUM OF IMMUNOLOGICAL ACTIVITY OF SYNTHETIC PEPTID THE ACTIVE CENTRE OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) AS A BASIS FOR THE EMPOWERMENT OF CREATING COSMETICS WITH A NEW GENERATION OF COMBINED EFFECTS
Abstract
In experiments in vitro and in vivo it was shown that a synthetic peptide of the active site of GM-CSF -ZP2 (chemical formula: THR NLE NLE ALA SER HIS TYR LYS GLN HIS CYS PRO) stimulates the activity of immune cells (lymphocytes, stem cells, neutrophils, monocytes), inhibits the growth, reproduction and biofilm formation of gram-positive and gram-negative bacteria and has antiviral activity against DNA and RNA viruses and activates the repair of damaged tissues of the organism at the wound process. The presence at this synthetic peptide of such a wide range of immunological effects (immunostimulatory, antibacterial, antiviral, and repair activity) determines the possibility of its use as the basis for creation of new effective medicinal and cosmetic preparations with combined properties that were implemented in the creation of cosmetics ACEGRAM.
Russian Journal of Immunology. 2017;20(3):377-380
377-380
FEATURES OF FORMATION OF THE MUCOSAL IMMUNITY OF THE UPPER RESPIRATORY TRACT IN PRETERM INFANTS IN THE FIRST YEAR OF LIFE
Abstract
Studied parameters of local immunity of the mucous membranes of the upper respiratory tract in 47 children the first year of life, born premature. Found that in preterm infants on nasal mucosa there is transient deficiency of IgG, low levels of IgE antibodies, cytokine IF-γ in the second half of life. These features of local immunity of the nasal mucosa is most pronounced in children born at gestational age of 28-32 weeks. The tension of mechanisms of the immune response in the mucosa of the oropharynx shows the excess production of proinflammatory cytokine IL-φ throughout the first year of life, and high IgE and IgA in the first half of the year.
Russian Journal of Immunology. 2017;20(3):381-383
381-383
CLINICAL ESTIMATION OF PROTECTIVE PROPERTIES OF PROBIOTIC PREPARATIONS ON BIOCHEMICAL INDICATORS OF BLOOD OF LABORATORY ANIMALS IN IRON INTOXICATION
Abstract
The effect of iron ions on biochemical blood indices was evaluated to study the effectiveness of probiotic preparations on the basis of bacteria of the genus Bacillus in the experimental intoxication of laboratory animals with iron. It has been established that a single oral administration of ferrous sulfate leads to an increase in the studied biochemical parameters in experimental groups with the addition of metal.
Russian Journal of Immunology. 2017;20(3):383-385
383-385
385-387
DYNAMICS OF INDEXES OF THE IMMUNOGRAM AT ATHLETES WITH DATUM LEVEL OF THE ENTROPY OF THE LEUKOCYTIC BLOOD COUNT
Abstract
At athletes with mainly aerobic power supply of motive activity and datum level of the relative index of an entropy of a leukocytic blood count the essential fluctuations of indexes of an immunogram in an annual cycle of preparation caused by impact of a rhythm of exercise stresses on process of adaptation of an organism to seasonal changes of conditions of the environment are shown.
Russian Journal of Immunology. 2017;20(3):387-389
387-389
THE INFLAMMATORY PATTERNS IN PATIRNTS WITH BRONCHIAL ASTHMA AND CONCOMITENT OBESITY
Abstract
Currently, it exists a firm tendency of increased prevalence of the excess body mass and obesity all over the world. In serum samples of 108 children and 51 adults with allergic diseases and bronchial asthma of different degree of severity, we measured the levels of ORP, total IgE, leptin, cytokines and IgE Abs against a number of self-tissue proteins by ELISA method. It was revealed the association between body mass index (BMI) and the clinical manifestations of allergy among children, that were characterizes by 2 times higher frequency of excess BMI in asthma group as compared to children with allergic rhinitis (AR) and atopic dermatitis (AD), without BA. It appeared that the elevation of BMI was also associated with worsening of asthma severity degree and the expanding the range of concomitant diseases. Besides of the enhanced levels of total IgE it was estimated the higher frequency of sIgE-response to environmental Ags. In both groups of adults and children, the obesity was associated with increasing of CRP, leptin and TNF-α in serum, being most enhanced in asthma group. The revealed associations confirm the link between obesity, as a chronic inflammatory condition with atopy and development of asthma.
Russian Journal of Immunology. 2017;20(3):390-393
390-393
CLINICAL-LABORATORY CHANGES GIRLS-TEENAGERS WITH VIOLATIONS OF THE MENSTRUAL CYCLE LIVING IN TERRITORIES WITH VARIOUS ANTHROPOGENIC LOAD
Abstract
We examined 266 patients 15-17 years old with menstrual disorders. Prevalence of oligomenorrhea in the Eastern zone, which is unfavorable environment. The levels of relative and absolute СD3+, СD4+ and СD8+ contents, the absolute amount of СD19+, FP, IgA, IgG, elevations of cortisol, testosterone, FSH, LH, and 17-OH-progesterone have been decreased. Indicators show the impact of the anthropogenic load on gynecological morbidity.
Russian Journal of Immunology. 2017;20(3):394-396
394-396
IMMUNOTROPIC INFLUENCE OF RHODOCOCCUS-BIOSURFACTANT IN VIVO
Abstract
Microbial biosurfactant complex Rhodococcus ruber IEGM 231 has immunoregulatory properties. It was established that the glycolipid complex and its dominant fraction of monoacyltrehalose exerted a depressing effect on innate and acquired immunity of laboratory mice with intraperitoneal and intramuscular injection.
Russian Journal of Immunology. 2017;20(3):396-398
396-398
PREVALENCE OF ANTINUCLEAR ANTIBODY IN CHILDREN WITH REACTIVE ARTHRITIS
Abstract
Children with reactive arthritis (n=26) of 2-17 years old were examinated. Antinuclear antibodies were determined by indirect immunofluorescence assay, antibodies to extractable nuclear antigens were defined by ELISA. It was found that 12 patients (46 %) with reactive arthritis had diagnostic titers of antinuclear antibodies detected by indirect immunofluorescence assay. Homogeneous and speckled fluorescence patterns were observed in patients with reactive arthritis. The prevalence of antibodies to extractable nuclear antigens in children with reactive arthritis was low (8 %).
Russian Journal of Immunology. 2017;20(3):398-400
398-400
EVALUATION OF THE SYSTEM OF HEMOSTASIS AND FUNCTIONAL ACTIVITY OF NEUTROPHILS IN PATIENTS WITH ATHEROSCLEROSIS OF UNPAIRED VISCERAL BRANCHES OF THE ABDOMINAL AORTA AND TYPE 2 DIABETES MELLITUS
Abstract
Aim. To study the features of the system of hemostasis and the functional activity of neutrophils in patients with atherosclerosis of unpaired visceral branches of the abdominal aorta, depending on the presence of type 2 diabetes. Methods. The study included 87 patients. The first group included 32 patients with atherosclerosis of vessels of the plannonic pool and type 2 diabetes, to the second group of 55 patients without type 2 diabetes. Results. The median activity of Wf in the group of patients with type 2 diabetes was 193,5 %, significantly exceeding the parameters of the second group of patients 160,0 %, (p = 0,002). The phagocytic number of neutrophils of the group of patients with type 2 diabetes significantly exceeded the similar index of the second group of patients (p = 0,01). Рositive correlation was found between the activity of von Wf and the intensity of phagocytosis of peripheral blood neutrophils (r =0,723; p=0,04), and phagocytic number (r=,771, p=0,025). Conclusion. In patients with atherosclerosis of the visceral branches of the abdominal aorta and type 2 diabetes, higher values of the phagocytic number of peripheral blood neutrophils and Wf activity were revealed.
Russian Journal of Immunology. 2017;20(3):400-402
400-402
RELATIONSHIP SERUM LEVELS AND GENE POLYMORPHISMS OF IL-1a, IL-1RA WITH EFFICIENCY OF IN VITRO FERTILIZATION (IVF)
Abstract
Women with tubal peritoneal infertility with an effective IVF had concentrations of IL-1a, IL-1ra in serum lower that women ineffective IVF. Genotype T/T gene IL-1a rs1800587 5'UTR area of the gene is associated with approach of pregnancy as a result of IVF (OR = 85,3, 95 %CI: 10,6-687, p<0,05).
Russian Journal of Immunology. 2017;20(3):402-404
402-404
CHANGES OF THE LEUKOCYTES QUANTITY, CONCENTRATION OF SERUM TOTAL PROTEIN AND ALBUMIN OF BLOOD UNDER EXPERIMENTAL IMMUNOSUPRESSION
Abstract
It was made a model for studying in vivo induced immunosuppression, which often occurs with secondary immunodeficiencies. In the process of its creation, the activity of phagocytic blood cells was studied, as well as the characteristics of the cytokine profile. However, little is known about the biochemical parameters of peripheral blood. The conducted studies showed that when induced immunosuppression as a result of changes in the activity of mononuclear cells, the content of the total protein in the serum of peripheral blood increases.
Russian Journal of Immunology. 2017;20(3):404-406
404-406
ANALYSIS OF THE FREQUENCY OF POLYMORPHIC VARIANTS OF TNF-α AND IL-10 GENES IN MISCARRIAGE THE FIRST TRIMESTER
Abstract
The study of the distribution frequency of polymorphic variants of genes TNF - α and IL-10 in miscarriage, the first trimester. It is found that the carriers of allele A of the gene of TNF-α risk of pregnancy in urogenital infections is higher and the GG genotype is protective, which contributes to a more favorable course of pregnancy.
Russian Journal of Immunology. 2017;20(3):406-408
406-408
EVALUATION OF CYTOKINE BALANCE OF AMNIOTIC FLUID IN PREGNANT WOMEN WITH PLACENTAL INSUFFICIENCY IN EARLY AND DELAYED MANIFESTATION OF CEREBRAL PATHOLOGY IN THEIR NEWBORNS
Abstract
The level of pro- and anti-inflammatory cytokines in the amniotic fluid of pregnant women with chronic placental insufficiency was determined. Immunological criteria for the timing of the manifestation of cerebral pathology in their newborns have been determined.
Russian Journal of Immunology. 2017;20(3):408-410
408-410
IMMUNOLOGICAL PARAMETERS CERVICAL SECRETION, DEPENDING ON THE COMPOSITION OF THE MICROBIOME OF REPRODUCTIVE TRACT OF WOMEN
Abstract
A comparative analysis of the secretory products of neutrophils in the reproductive tract of women with chronic inflammatory diseases caused by opportunistic microorganisms. Shows the different levels of the secretory peptides depending on the composition of microbial associations. Studied indicators of the level of immunoglobulins in the cervical secretion when combined PVI with opportunistic microorganisms.
Russian Journal of Immunology. 2017;20(3):410-412
410-412
FEATURES OF DIFFERENTIATION OF DENDRITIC CELLS IN TYMUS IN CHILDREN DEPENDING ON TYPE OF CYANOSIS IN CONGENITAL HEART DISEASES
Abstract
The specific features of differentiation of dendritic cells (DC) of the thymus in children with depending on the type of cyanosis at congenital heart diseases (CHD) have been studied. It has been established that the accumulation of DC in the medullar and cortical compartment of the thymus occurs at CHD, especially in the case of blue types of CHD. When comparing the blue and white types of CHD, a change in the content of myeloid and plasmacytoid DC of the thymus was revealed. Thus, hypoxia affects the differentiation of the DC at thymus at CHD.
Russian Journal of Immunology. 2017;20(3):413-415
413-415
HUMAN PAPILLOMAVIRUS INFECTION: А CYTOKINE IMBALANCE IN WOMEN AND MEN FROM COUPLES
Abstract
The study presents the results of the study of the most significant cytokines of cervical mucus in women and the ejaculate of 38 married couples with a papillomavirus infection. Changes in the levels of indicators are as one-sided, expressed in an increase in the number of IL-2, IL-10, sIgA, and versatile, characterized by a decrease in IFN-γ in women, with its normal level in men.
Russian Journal of Immunology. 2017;20(3):415-417
415-417
LOCAL AND SYSTEMIC INFLAMMATORY REACTIONS AFTER IMPLANTATION OF DIFFERENT COMPOSITION SMALL DIAMETER VASCULAR GRAFTS
Abstract
The most important criterion of implantable vascular prostheses biocompatibility is the intensity of local and systemic inflammatory reactions to the graft material. Purpose: To evaluate the intensity of local and systemic inflammatory reactions of the organism in the acute period after implantation of small diameter vascular prostheses created on the basis of different biodegradable polymers. Obtained by electrospinning biopolymer prosthesis were implanted in the abdominal aorta of rats: I - polycaprolactone (PCL); II - copolymer of polylactic acid/polycaprolactone (PLC 7015); III - copolymer of polylactic acid/ polycaprolactone with polyglycolide (PLC 7015-PGS); IV - polylactic acid/polycaprolactone (PL-18PCL); V - polycapro-lactone/polyglycolide (PCL-PGS); VI - sham-operated control group. Grafts explantation was performed on the 14th day. The intensity of the systemic inflammatory response was assessed by serum level of IL-6, IL-10 and MCP-1, the intensity of the local inflammatory reaction - by HE-stained grafts sections analyzing. Prosthesis implantation in all cases was accompanied by the local inflammatory reaction development that was most pronounced in the case of PL-18PCL and PCL-PGS. Elevated level of MCP-1 was observed only by PLC 7015 grafts implantation. All studied polymers could be used as a stock for small diameter vascular prostheses development.
Russian Journal of Immunology. 2017;20(3):417-419
417-419
THE IMMUNE MARKERS OF THE INCREASED RISK OF THE FREQUENT ACUTE RESPIRATORY VIRAL INFECTIONS IN WORKERS OF CHEMICAL PRODUCTION
Abstract
Method of assessment of high risk of frequent cases of SARS by data from immunoepidemiological cross-sectional study are proposed. The data of the immunological examination of the workers in the chemical enterprise who had contact with a production factor were used to test it. The results are discussed.
Russian Journal of Immunology. 2017;20(3):419-421
419-421
THE POSSIBLE ROLE OF MAST CELLS IN THE PATHOGENESIS OF SEVERE EXPERIMENTAL INFECTION IN MICE VACCINATED AGAINST INFLUENZA
Abstract
CBA mice were immunized parenterally with the NIBRG-14 vaccine strain of A/H5N1 influenza virus, and after 2 weeks they were infected with a high dose of the live IDCDC-RG2 strain of the A/H5N1 influenza virus. The course of the infection was severe and accompanied by high mortality. The injection of antihistamines (a mixtures of H1 and H2 histamine receptors blockers), at the time of infection, resulted in a 10% reduction in mortality (p<0.05). Data indicate that the severe course of influenza infection in vaccinated animals at least partially depends on the mast cells and histamine they release presumably upon activation by IgG antibodies and the virus.
Russian Journal of Immunology. 2017;20(3):422-424
422-424
PHENOMENON OF IMMUNOLOGICAL MEMORY DURING PREGNANCY
Abstract
In preterm delivery both in the 1and in the 3 trimesters of pregnancy there was an elevated level of ef-fectory memory cells in the subset of cytotoxic T lymphocytes. Thus, the preterm delivery was associated with the enhancement of CD8+ lymphocytes differentiation towards terminal stages.
Russian Journal of Immunology. 2017;20(3):425-426
425-426
ASSESSMENT OF RESISTANCE OF PRO-BIOTIC STRAINS OF MICROORGANISMS TO ESSENTIAL AND TOXIFEROUS HEAVY METALS
Abstract
Data on the experimental studying of influence of salts of heavy metals on growth of probiotic strains of bacteria of the sort Bacillus are presented in article. During the made experiments it was established that presence of various salts of heavy metals differently influences dynamics of body height of the studied microorganisms. But despite it, the common regularity in influence of the same ion of metal on all studied microorganisms is observed. Presence of ions of lead and iron has the stimulating effect on body height of all studied microorganisms, and presence of ions of a cobalt and cadmium oppresses them body height. Presence of ions of Zincum and manganese does not exert impact on dynamics of growth of the studied strains, the exception makes only B. licheniformis for which Zincum is a growth factor.
Russian Journal of Immunology. 2017;20(3):427-428
427-428
IMMUNOREACTIVITY OF PATIENTS WITH CHRONIC VIRAL HEPATITIS B AND C WITH THE DEVELOPMENT OF SYSTEMIC AUTOIMMUNIZATION
Abstract
Screening studies among 232 patients with chronic viral hepatitis B and C revealed systemic (organ-specific) autoantibodies in 43.8 % and 30.2 % of cases, respectively. When studying the characteristics of immune response of patients it was established that in case of chronic viral hepatitis B pathogenetic bases of development of autoimmune disorders of a systemic nature are the stimulation of production of antibodies by B lymphocytes, and a decrease in elimination of the pathogen, with a weakly expressed antiviral response. In chronic viral hepatitis C, autoimmune disorders of a systemic nature develop against a background of a deep T-cell immunodeficiency state and an inadequate antiviral response. And also with systemic autoantigenemia, along with high indicators of circulating immune complexes, which indicate activation of antiviral protection, there is a deficiency of phagocytic activity of neutrophils, which further aggravates the immunopathological process.
Russian Journal of Immunology. 2017;20(3):429-434
429-434
THE IMPORTANCE OF GENOTYPIC AND IMMUNOPHENOTYPIC CHARACTERISTICS IN THE PATHOGENESIS AND TREATMENT OF CHRONIC HCV-INFECTION
Abstract
The article is devoted to the investigation of the correspondence of the genotype of the IL-28B gene to the virological, biochemical and immunological parameters of patients with chronic viral hepatitis C in the course of antiviral therapy of pegylated interferon α and ribavirin. It was found that with the genotype of the CC gene of the IL-28B gene at the locus rsl2979860, in which the non-genotype of the hepatitis C virus was most often detected, self-healing and high efficacy of PVT are most often observed. On the part of the organism’s reactogenicity, this was accompanied by a more pronounced cytolytic syndrome and a high immunological reactivity of the organism, manifested in an increase in the expression of cytotoxic lymphocytes CD8+, activation markers CD23+ and CD38+.
Russian Journal of Immunology. 2017;20(3):435-439
435-439
RELATIONSHIP OF THE EFFICACY OF ULIPRISTAL ACETATE THERAPY WITH POPULATION SUBSETS OF ENDOMETRIAL MACROPHAGES OF PATIENTS WITH UTERINE LEIOMYOMA
Abstract
The decrease of the leiomyoma size under the influence of ulipristal acetate therapy took place in women with initially high level of endometrial TGFpi+ macrophages and with elevation of TGFpi+ and CD163+ macrophages after treatment. Lack of the treatment effect was associated with diminishment of M2 macrophages and increases the amount of IL-12+ macrophages.
Russian Journal of Immunology. 2017;20(3):440-442
440-442
442-444
ASSESSMENT OF HIGH LEVELS OF ANTIBODIES TO HERPES SIMPLEX VIRUS CLINICAL VALUE IN HEAD AND BACK PAIN OUTPATIENTS
Abstract
Aim: to assess clinical value of antibodies to Herpes Simplex Virus (HSV) in outpatients with head and back pain. Cross sectional study included 45 female outpatients aged from 40 to 60. Two or more HSVinfec-tion exacerbations per year were registered in 25 persons (Group1) while 20 women had no exacerbation during previous year (Group 2). General practitioner and neurologist examination have been performed accompanied by IEA IgG anti-HSV type 1,2 antibodies assessment. Results: Group 1 showed 5-fold higher IgG anti-HSV antibodies level and post-herpetic neuralgia frequency then Group 2. High IgG anti-HSV antibodies level was associated with increased odds of HSV infection exacerbation (OR = 1.8; 95 % СШ 1.2-2.6).
Russian Journal of Immunology. 2017;20(3):444-445
444-445
INDICATORS OF CELLULAR IMMUNITY IN PATIENTS WITH PURULENT WOUNDS OF LOWER EXTREMITIES TREATED WITH COMBINED APPLICATION OF MILIACILE AND EHF-THERAPY ON THE BACKGROUND OF DIABETES MELLITUS
Abstract
Therapeutic effectiveness of combined local application of Miliacile and EHF-therapy was studied in 22 patients with purulent wounds against the background of diabetes mellitus. 22 patients with similar purulent wounds were treated only with Miliacile, applied locally. The use of Miliacile, especially in combination with EHF-therapy, had positive influence on the dynamics of cellular immunity recovery in patients.
Russian Journal of Immunology. 2017;20(3):446-448
446-448
APPLICATION OF THERAPEUTIC COMPOSITION OF TOPICAL APPLICATION CONTAINING ACEGRAM AND SILATIVIT-GEL ON THE MODEL OF CHRONIC PERIODONTITIS IN RATS
Abstract
The research presents the results of evaluation of therapeutic effect on a new drug composition, containing the Acegram and Silativit-gel on the model of chronic periodontitis in rats. Objective evidence of the efficacy of the analyzed drug composition was obtained.
Russian Journal of Immunology. 2017;20(3):448-450
448-450
PROGNOSTIC CRITERIA FOR ONCOHEMATOLOGICAL DISEASES IN CHILDREN
Abstract
There are the results of studies of immunological status of pediatric patients with various oncohema-tological diseases: Hematological malignancies, solid tumors including associated with cytomegalovirus infection. The development of malignant process is accompanied by changes in the indices of cellular immunity. Expressed violations of immunological reactivity with a decrease in the indices of cytotoxic cells were observed in diseases associated with cytomegalovirus infections.
Russian Journal of Immunology. 2017;20(3):450-451
450-451
EFFECT OF ERYTHROPOIETIN ON PARAMETERS OF CELLULAR IMMUNITY IN PRETERM INFANTS OF EARLY PRETERM BIRTH USING THE SYSTEM CYTODIFF
Abstract
Aim of the study: to investigate the effect of erythropoietin (EPO) on the parameters of cellular immunity in preterm infants (PI) of early preterm birth (EPB). The study was performed on 15 full-term infants and 25 PI from EPB (gestational age 28 weeks - 30 weeks and 6 days). EPO was applied 6 days of life at a dose of 200 IU/kg every 48 h. Number of subpopulations of leukocytes in blood was determined by flow cytometry with antibodies of system “CytoDiff”. It was found that PI from EPB at 8 and 18 days of life reduced the representation in blood of B-lymphocytes, T-helper cells, T - and NK-cells; on the 8th day of life increases the number of monocytes and their precursors decreases the number of eosinophils; 18 day - reduced the number of monocytes, mature neutrophils, increases the number of mast cells, precursors of B-and T-lymphocytes. The use of EPO leads on the 8 days of life to increase in blood the total number of lymphocytes due to T - and NK-cells, the number of immature granulocytes; 18 day - to increase the total number of lymphocytes at the expense of B-lymphocytes, T-helper cells, T - and NK-cells, increasing the number of mature neutrophils, immature granulocytes, the precursors of monocytes.
Russian Journal of Immunology. 2017;20(3):452-454
452-454
STATE OF THE IMMUNE STATUS OF CHILDREN LIVING IN CONDITIONS OF AEROGENIC EXPOSURE TO METALS
Abstract
It has been demonstrated that among the child population, living under exposure to aluminium, more than 40 % of children have a significant (p<0.05) decrease relative to the norm of phagocytic activity, as well as a significant change (p <0.05) in the CD- immunogram- oppression Annexin V-FITC+7AAD negative cells, СD95+, Bax, Bcl-2 protein (from 57 to 73 % of children) and 56 % of children have an activation of СD127. The IgG testing detected a specific sensitization of a body in relation of the reference value and the control group index by 1.5 time.
Russian Journal of Immunology. 2017;20(3):455-457
455-457
INDICATORS OF INNATE AND ADAPTIVE IMMUNITY IN EXPERIMENTAL THERMAL INJURY IN THE APPLICATION OF TRANSDERMAL FILMS WITH ERYTHROPOIETIN
Abstract
The aim of this work is to investigate the effect of transdermal film (TDF) with erythropoietin (EPO) on the parameters of innate and adaptive immunity in experimental thermal injury (TI). The experiment was carried out on 70 white nonlinear Mature rats, which were divided into 3 groups: 1 (n=10) - intact control, 2 (n=30) -TI, 3 (n=30) -TI with the imposition of TDF with EPO. TI IIIA degree with an area of 3.5 % was modeling by immersion of the site interscapular region of the animal in clean water with a temperature of 98-99°С for 12 s. the Study was performed at 3rd, 8th and 14th days TI. The number of neutrophils in the blood was determined by hematological analyzer “Medonic - M 16” (Sweden). The absorption ability of neutrophils blood was examined using of latex particles, the oxygen-dependent metabolism in spontaneous and induced NST-test. With the help of a flow cytometer “Navios” in the blood determined the number of CD3+ and CD45RA+ lymphocytes. The death of lymphocytes was assessed by staining cells with fluorochrome conjugated with annexin V (Annexin-5-FITC) and 7-aminoantipyrine D (7-AAD). It was found that experimental TI in the blood increases the number of neutrophils, their phagocytic and NST-reducing ability, reduced number of CD3+ and CD45RA+ lymphocytes at 5th, 8th and 14th days, increases in blood lymphocyte count with evidence of apoptosis and/or necrosis at 3rd and 8th days. The use of EPO in the composition of TPF in the TI did not accompanied by a change in the number of neutrophils in the blood at 3rd, 8th and 14th days, leads to a decrease in phagocytosis activity at day 14th, the intensity of phagocytosis - on the 8th day, activity and the intensity of spontaneous NST-test at day 14th, the induced NST-test - 3rd, 8th and 14th days; increase of CD3+ lymphocytes in the blood on the 8th day, the reduction in the number of blood lymphocytes with signs of apoptosis and/or necrosis at 14th day.
Russian Journal of Immunology. 2017;20(3):457-460
457-460
FUNCTIONAL CONDITION OF A PERIPHERAL LEUCOCYTAL SERIES OF THE BLOOD AT THE STUDENTS OF MEDICAL UNIVERSITY WHO ARE GOING IN FOR PHYSICAL CULTURE
Abstract
In this work results of a research of a functional condition of a peripheral leucocytal link of a blood at the students who are studying at the first and second courses of medical university and sectioned into 3 groups are presented. The first group was made by the students belonging to I and II groups of health and, studying according to the main program of physical training; the second group - athletes-volleyball players; the third group students made III and IV groups of health studying according to the medical physical culture program. At the end of the first year at students of the first group indicators of a leucocytal link didn’t differ from control sizes, and after the second year of training all indicators were enlarged in comparison with indicators of the first year of training. At students of the II group (athletes) the reliable augmentation of all indicators of a leukocyte series of a blood on the second year of training was recorded. In the third group on the second year of training, engaged according to the medical physical culture program, reliable differences of indicators of a leucocytal link of a blood in comparison with students of the main group, demonstrating to what testifies weren’t taped: occupations by physical culture promote conservation of normal functioning of immune system.
Russian Journal of Immunology. 2017;20(3):461-463
461-463
PECULIARITIES OF IMMUNOLOGICAL RESPONCE DURING LIVER CIRRHOSIS DEPENDING ON SEVERITY LEVEL
Abstract
It has been established that the level of IL-6 in patients with subcompensated CP (class B) was significantly higher than in the compensated stage (p <0.001), and also had significant differences in patients with subcompensated and decompensated CP (p <0.001). The concentrations of VEGF and G-CSF significantly increased at the stage of severe cirrhosis (p <0.001 and p = 0.04, respectively). Proinflammatory cytokine TNF-α and anti-inflammatory IL-4 significantly increased during transition to the subcompensated stage of the disease (р=0.01 and р=0.04, respectively). There are no reliable differences between allelic variations of polymorphic gene loci TNF (G4682A), IL-6 (C174G) and VEGFA (G-634C), associated with liver cirrhosis severity levels, detected by us.
Russian Journal of Immunology. 2017;20(3):463-465
463-465
SPONTANEOUS AND PHYTOGEMAGGLUTININ-STIMULATED EXPRESSION OF ACTIVATION MARKERS OD25, OD38, OD69 ON T-LYMPHOCYTES IN CHILDREN WITH DIFFERENT PATHOLOGY
Abstract
Children with systemic lupus erithematosus, juvenile arthritis, reactive arthritis, chronicle hepatitis C and conditionally healthy children were investigated. Spontaneous number of СD3+СD25+ in children with hepatitis and number of СD3+СD69+ in patients with rheumatic diseases were similar to the control, after stimulation by phytogemagglutinin the amount of these subpopulations was less, then in healthy children.
Russian Journal of Immunology. 2017;20(3):465-467
465-467
CHARACTERIZATION OF ANTIMICROBIAL PEPTIDES FROM DOMESTIC HORSE'S PLATELETS
Abstract
The aim of this work was to investigate an antimicrobial properties of platelets of domestic horse (Equus ferus caballus) and isolate an antimicrobial components. Using a liquid chromatography technique (RP-HPLC) ten fractions were collected from platelet's acid extract. Among these a four fractions demonstrated antimicrobial activity against S.aureus.
Russian Journal of Immunology. 2017;20(3):468-469
468-469
THE USE OF RONCOLEUKIN FOR THE CORRECTION OF THE SECONDARY IMMUNODEFICIENCY IN THE ELDERLY PATIENTS WITH ACUTE CHOLECYSTITIS
Abstract
In this study the analysis of the immune status of the elderly patients with acute cholecystitis was carried out before surgery and on the 7th day afterwards. A reduction in the number of T-lymphocytes was found before surgery. On the 7th day after surgery, the down-regulation of the T-cell immunity was observed that manifested by the twofold decrease in the populations of T-helpers and T-killers. The use of immunomodulatory drug roncoleukin during first days after surgery allowed to increase the number of T-lymphocytes and thus to prevent the progression of secondary immunodeficiency in the elderly patients.
Russian Journal of Immunology. 2017;20(3):470-471
470-471
EVALUATION OF THE IMPACT OF SECONDHAND SMOKE DURING PREGNANCY ON INFANT WISTAR RATS
Abstract
Infant rats born from Wistar females who were exposed to secondhand smoke throughout pregnancy showed the decreased parameters of sensomotor and somatic growth, disorders of structural and functional activity and low number of thymocytes and splenocytes.
Russian Journal of Immunology. 2017;20(3):471-473
471-473
MEASLES DIAGNOSTICS: PROBLEMS AND MODERN APPROACHES
Abstract
Key methods of measles diagnostics are presented in the paper. Immunoenzyme, chemiluminiscent assays and polymerase chain reaction are the most effective modern measles diagnostic methods. Development of more perfect diagnostic techniques is essential component of WHO Global measles and rubella strategic plan.
Russian Journal of Immunology. 2017;20(3):474-476
474-476
INVESTIGATION OF GROWTH AND VIABILITY OF HYBRIDOMA BAP3 CELLS IN DIFFERENT CULTURE MEDIA IN VITRO
Abstract
Growth and viability in different culture media after thawing of hybridoma BAP3 cells that produces monoclonal antibodies against pregnancy-specific b1-glycoprotein (PSG) were studied in vitro. We show that cultivation in [DMEM+12 % FCS] leads to maximal cell growth (1,8-fold in two days) with retention of viability of more than 70 %. There were 1,1-1,2-fold growth and viability of 55-59 % in [DCCM-1]-based media and 1,6-fold growth in two days in [RPMI-1640+12 % FCS]. Therefore, application of [DMEM+12 % FCS] medium seems to be the most appropriate for cultivation of hybridoma BAP3 cells after their thawing from cryopreservation.
Russian Journal of Immunology. 2017;20(3):476-478
476-478
INVESTIGATION OF BIOSPECIFIC INTERACTION BETWEEN DNA-APTAMER AND CARBON NANOPARTICLES
Abstract
Aptamers are DNA- and RNA-oligonucleotides that can selectively bind target molecules. Objective of our work was to investigate into interaction of biotinylated DNA-aptamer specific to human IgE with carbon nanoparticles covalently conjugated with streptavidin. Maximal level of specific interaction (about 40 pM per 1 mg of nanoparticles) was observed when concentration of aptamer was 100 nM.
Russian Journal of Immunology. 2017;20(3):478-480
478-480
FUNCTIONAL ACTIVITY OF MONOCYTES IN PREMATURE INFANTS IN NEONATAL DYNAMICS
Abstract
To assess the functional activity of monocytes in preterm infants of different gestational age (22-27.6 weeks and 28-31 weeks) in the dynamics of the neonatal period, cord and peripheral blood were examined. All children with an ENMT at birth had a decreased willingness of effector cells to participate in intercellular interactions (decrease in the number of CD14+ CB 282+ and CD14+ CD157+ monocytes), in infants of gestational age 22-27 weeks - to antigen presentation of the pathogen (decrease in HLA receptor expression -DR+). At the end of the neonatal period, the functional activity of monocytes is restored, the number of CD14+ HLA-DR+ cells exceeds those of full-term children.
Russian Journal of Immunology. 2017;20(3):480-482
480-482
APOPTOSIS OF MONOCYTES, THEIR METABOLIC FUNCTIONS AND CYTOKINE PROFILE, WITH LIMITED AND SYSTEMIC SCLERODERMA
Abstract
With systemic scleroderma demonstrated activation of apoptosis of monocytes, their metabolic functions against the background of increasing levels of proinflammatory cytokines. Limited scleroderma significant changes in rates of apoptosis of monocytes, their metabolic functions of the profile was not found on the background of the increasing of proinflammatory cytokines.
Russian Journal of Immunology. 2017;20(3):482-484
482-484
COMPARATIVE ANALYSIS IMMUNOTROPIC EFFECTS IN VITRO DERINAT AND SYNTHETIC TLR 9 AGONIST ON RECEPTOR FUNCTION OF MONOCYTES IN NORMAL AND INFECTIOUS PROCESS
Abstract
Comparative evaluation of character of the expression of TLR 9, CD11b and CD14 receptors monocytes whole blood under the influence of Derinat and synthetic TLR 9 ligand in healthy individuals and in acute infectious diseases of viral and bacterial origin has been studied. It has been shown that the unidirectional effects of study drugs relate changes in the number of monocytes only in groups with infectious process. Differently directed effects identified in relation to expression of molecules QD11b monocytes in all groups.
Russian Journal of Immunology. 2017;20(3):485-487
485-487
SEMAPHORIN 3A INHIBITS THYMOCYTE FUNCTIONS
Abstract
Semaphorin 3A (Sema 3A) is known to be an axon guidance molecule and is implicated in the development of nervous system. The aim of the study was to evaluate the influence of Sema 3A on functions of murine thymocytes, such as: proliferation, migration and adhesion to thymic epithelial cells. Sema 3A suppressed thymocyte proliferation at 100 and 200 ng/ml, possessed a chemorepellent activity towards thymocytes in the transwell system, and inhibited thymocyte adhesion to murine thymic epithelial cell lines cTEC 1-2 and mTEC 3-10. Thus, Sema 3A is a negative regulator of important thymocyte functions and may essentially affect the process of thymocyte maturation.
Russian Journal of Immunology. 2017;20(3):487-489
487-489
489-491
491-493
MOLECULAR DIAGNOSTICS OF IGE REACTIVITY TO BIRCH POLLEN ALLERGENS IN CHILDREN WITH ALLERGIC RHINITIS
Abstract
The aim of this study was to detect sIgE to isoallergens of birch pollen and to evaluate a contribution of some homologous food allergens to the development of sensibility in AR patients. There were 21 blood sera of AR patients and 20 blood sera of healthy persons without allergy symptoms. The total IgE levels and sIgE to allergens were measured by the ImmunoCAP method (Phadia, Sweden), as well as by the ELISA kits (Alkorbio, Russia). We detected high total IgE levels, sIgE to allergens of birch pollen, apple, celery, as well as to recombinant allergens of birch Bet v 1, Bet v 2 and soybean - Gly m 4 in AR patients. Correlation analysis showed the direct valid dependence between the sIgE levels to birch isoallergen Bet v 1 and soy isoallergen Gly m 4 (r=0,84; p<0,05). There was also the direct valid correlation between the sIgE levels to birch isoallergen Bet v 1 and allergens of apple and celery (r=0,67 and 0,57, accordingly). Our finding permits assert that cross-reactivity can be important in exacerbation of clinical symptoms in AR patients including progress of oral allergy syndrome.
Russian Journal of Immunology. 2017;20(3):494-496
494-496
SPECIFICITY OF PERIPHERAL BLOOD PARAMETERS DEPENDING ON THE LEVEL OF SPORTS QUALIFICATION OF SPORTSMEN
Abstract
The investigation is aimed to problems of dynamic changes associated with a progress in sports qualification of sports professionals, and changes in peripheral red blood cell-associated parameters, probability corresponding to positive or negative signs of blood system adaptation to aerobic or anaerobic physical activity conditions. The optimal level of red blood cell-associated parameters, that meets the elevated oxygen tissue consumption demands, was observed in groups of Masters of sports and candidates for Master of sports. In groups of wrestlers and boxers some variations in red blood cell-associated parameters were also observed, with mostly lack of statistically significant differences between sports, sport qualification, or other parameters investigated, indicating no or little impact of sports specificity factors on red blood cell-associated parameters. The values of “leucocytes blood formula entropy” in groups of skiers, swimmers and wrestlers were within normal range, thus indicating a relatively adequate state of adaptation of blood system and it’s regulation in response to increased physical activity, but in boxers group they were out of normal range, probably due to negative side effect of adaptation processes or even a pre-morbid (pre-disease) state. The values of “integral coefficient of blood deterioration” also indicated on worse changes, being statistically significant in group of boxers only, thus reflecting deviations of functional activity, probably due to negative adaptive reaction accumulation as a result of specific factors in this sport. Conclusions: Progress in sports qualification mostly of aerobic (cross-country skiers, swimmers) or anaerobic (wrestlers, boxers) physical activity, was accompanied with changes in red blood cell-associated parameters, thus with sufficient probability corresponding to specific effects of aerobic or anaerobic physical activity on sportsmen.
Russian Journal of Immunology. 2017;20(3):496-498
496-498
TO THE QUESTION OF DETECTION OF HEMOPHAGOCYTIC MACROPHAGES IN PERIPHERAL BLOOD OF NEWBORNS WITH THE MULTIPLE ORGAN DYSFUNCTION SYNDROME
Abstract
The phenomenon of detection of hemophagocytic macrophages in newborns with multiple organ dysfunction syndrome (MODS) was studied. It has been shown that detection of hemophagocytic macrophages in peripheral blood takes place in newborns with severe manifestations of MODS and is a prog-nostically unfavorable sign.
Russian Journal of Immunology. 2017;20(3):499-501
499-501
INDUCTION OF ANTIGENIC DETERMINANTS FOR REGULATORY RHEUMATHOID FACTOR ON FC FRAGMENTS OF HUMAN IgG
Abstract
Recently, we detected a rheumatoid factor, called regulatory, whose production prevents development and causes a remission of experimentally-induced autoimmune diseases. The aim of this study was to elucidate the mechanism of induction of antigenic determinants for regulatory rheumatoid factor onIgG and Fc fragments of human IgG. It was found out that antigenic determinants for regulatory rheumatoid factor are formed in the hinge region of IgG uponthe reduction of sulfhydryl bondby treatment of the IgG molecule with polyethylene glycol or dithiothreitol. In response to the immunization of rats with native human IgG, the production of antibodies similar in specificity to human regulatory rheumatoid factor occurs, suggests that there is an endogenous mechanism for the formation of neoantigenic determinants for regulatory rheumatoid factor
Russian Journal of Immunology. 2017;20(3):501-503
501-503
CLINICAL EXPERIENCE OF USE OF THE PRO-BIOTIC MEDICINE "SPOROBAKTERIN" AT INTOXICATION LEAD
Abstract
Clinical experience showed that use of the probiotic medicine “Sporobakterin” promotes not only the fissile elimination of ions of lead from an organism to 58,9 % (from an integument to 67,9 % muscular tissue to 47,1 % and a bone tissue to 61,5 %), but also prevents development of degenerative changes of internals that in our opinion has direct dependence. On by it application of Sporobakterin at treatment of intoxication lead interferes with development of inflammatory process in an organism what morphological, hematological and biochemical indexes of blood of the experimental animals testify to, and also have the stimulating effect of factors of nonspecific resistance of an organism.
Russian Journal of Immunology. 2017;20(3):503-505
503-505
MILIACIN EFFECT ON IMMUNOLOGICAL PARAMETERS IN CASE OF EXPERIMENTAL SEPSIS
Abstract
Administration of miliacin to mice (CBA x C57Bl6)F1 with two types of generalized infection (caused by P. aerugenosa or K. pneumoniae strains) showed positive dynamics of cell populations in immunogen-esis, metabolic activity of immune peritoneal macrophages and limited production of IL-17 splenocytes.
Russian Journal of Immunology. 2017;20(3):506-508
506-508
ASSESSMENT OF SYSTEMIC INFLAMMATORY RESPONSE INDICATORS AS PREDICTORS FOR CHRONIC RENAL ALLOGRAFT DYSFUNCTION
Abstract
The article present the results of a study of the diagnostic efficacy of various SIR indicators (IL-6, IL-8, IL-10, TNFa, CRP), duration of dialysis before renal transplantation, post-allotransplantation period and HLA mismatch score (the number of mismatches from 6 antigens HLA loci A, B, DR) with respect to the development of chronic renal allograft dysfunction (CRAD). It was shown that the level of proinflamma-tory cytokine TNFa is a promising marker for the prognosis of the CRAD development.
Russian Journal of Immunology. 2017;20(3):508-510
508-510
THE REGULATION OF FAS-DEPENDENT APOPTOSIS IN RSA PREGNANCY
Abstract
Development of the threatened abortion at the first trimester of the pregnancy in women with recurrent miscarriage is accompanied by the suppression of the FasL-depended apoptosis-inducing activity of peripheral blood lymphocytes and monocytes which is correlated with the degree of clinical symptoms and the presence of the markers of bacterial and viral infection.
Russian Journal of Immunology. 2017;20(3):510-512
510-512
CHANGES OF IMMUNE REGULATORY MARKERS IN CHILDREN'S POPULATION UNDER CONDITIONS OF INDUSTRIAL POLLUTION BY METALS
Abstract
A study of immune regulation in children living in the region of industrial pollution by aluminum and other metals showed a decrease in phagocytic activity, expression of CD95 T-cell receptors, increased production of leukotrienes C4/D4/E4 and specific IgG to aluminum.
Russian Journal of Immunology. 2017;20(3):512-514
512-514
DISTRIBUTION OF TWO-LOCUS HAPLOTYPES OF HLA-DRB1-TNFA-308 IN PATIENTS WITH ULCERATIVE COLITIS OF THE RUSSIAN POPULATION OF CHELYABINSK REGION
Abstract
The analysis of the distribution of HLA-DRB1-TNFA-308 two-locus haplotypes frequencies in patients with ulcerative colitis (UC) of the Russian population of the Chelyabinsk region is carried out in this article. The 19 haplotypes were identified, among which 4 haplotypes, containing -308*G TNFA allele, were characterized by high incidence rates in groups of UC patients and healthy individuals. A peculiarity of the UC patient group was the presence of two haplotypes containing the rare allele with the substitution -308*A TNFA: HLA DRB1* 01-TNFA-308*A; HLA DRB1* 13 -TNFA-308*A. These haplotypes were not found in the comparison group of healthy individuals. These haplotypes can be considered as markers of susceptibility to ulcerative colitis in the Russian Chelyabinsk region.
Russian Journal of Immunology. 2017;20(3):514-516
514-516
FACTORS OF INNATE IMMUNITY IN WOMEN WITH ABDOMINAL OBESITY AND OVERWEIGHT, THEIR RELATIONSHIP WITH PARAMETRES OF CARBOHYDRATE AND LIPID METABOLISM
Abstract
The study evaluated the state of innate immunity in young women with abdominal obesity and overweight. In women with isolated abdominal obesity the activation of neutrophil function was observed. During the progression of obesity in young women the decreasing of parameters of phagocytosis was revealed. It was associated with increased volume of abdominal fat tissue and disorders of lipid and carbohydrate metabolism.
Russian Journal of Immunology. 2017;20(3):517-519
517-519
ANTIGENPRESENTAL FUNCTION OF B-CELLS IN PATIENTS WITH REMITTING MULTIPLE SCLEROSIS
Abstract
The antigen-presenting function of B-lymphocytes in patients with a remitting form of multiple sclerosis was studied. It was found that B cells in the presentation of different antigens have a different ability to induce differentiation of naive T cells in Th17 and Treg. A new mechanism for activating the differentiation of naive T cells in Th17 in the presentation of autoantigens with B cells in multiple sclerosis has been suggested.
Russian Journal of Immunology. 2017;20(3):519-521
519-521
LOCAL IMMUNITY OF SKIN AS REFLECTION OF ITS AGE AND FUNCTIONAL STATE
Abstract
The functional condition of skin directly coreesponds to its appearance. The cooMinated woA of the main cells of skin defines ability to support a homeostasis of skin and to react to influence of external and internal environment. Age dynamics of separate “immunocytes of skin” is traced (T, B- lymphocytes, monocytes/macrophages, co^ulent cages). External characteristics of skin have distinct collation communications with the studied parameter. Apparently, there is a possibility of influence on appearance of skin by impact on resident immunocompetent cells of skin.
Russian Journal of Immunology. 2017;20(3):521-523
521-523
CHARACTERISTICS OF SECRETORY IMMUNE SYSTEM IN WOMEN AND MEN INFECTED WITH HPV HIGH-RISK TYPES, DEPENDING ON VIRAL LOAD
Abstract
Human papillomavirus infection (HPV infection) is equally widespread among women and among men. The purpose of this study was to determine the levels of main immunological parameters of the secretory immune system in women and men from 38 couples infected with HPV of high oncogenic risk, with different types of viral load. The object of the study in women was cervical mucus, men - ejaculate. Results. Both women and men from couples with HPV each time a viral load was determined by the increase in the number of IL-2 and IL-8, decrease of IL-4 and TNF-α. When significant concentrations of HPV DNA in women they have increased the number of sIgA, men elevated were IL-φ, IFN-α and IFN-γ.
Russian Journal of Immunology. 2017;20(3):524-526
524-526
IMMUNOLOGIC FEATURES OF THE PURULENT-NECROTIC COMPLICATIONS OF THE DIABETIC FOOT SYNDROME
Abstract
Character of immune infringements essentially depends on pathogenetic variant of the diabetic foot syndrome and its severity degree. Results confirm pathogenetic heterogeneity of clinical forms of complicated diabetic foot syndrome and demand the differentiated approaches to treatment.
Russian Journal of Immunology. 2017;20(3):526-528
526-528
INTRASPECIFIC AND INTERSPECIFIC PATTERNS OF MICROBIOTA - INFLAMMATORY HOST POTENTIAL INTERACTIONS IN CHILDREN WITH ASD
Abstract
Analysis of correlation links of intraspecies patterns - “microbe - microbe”, “microbe - endotoxin”, inflammatory potential of the host “cytokine - cytokine” and interspecies - “microbe - host” demonstrates close relationships between members of the small intestine microbiota community in healthy children and in children with ASD. Statistically significant correlations of the pattern of the inflammatory potential of the cytokine-cytokine host and of the pattern of interspecies links “microbe - host” were registered only in the group of children with ASD.
Russian Journal of Immunology. 2017;20(3):529-531
529-531
LUNG SURFACTANT AND PHAGOCYTE ACTIVITY OF MACROPHAGES IN NEUROIMMUNE IMPACT ON BLACK SUBSTANCE OF THE BRAIN
Abstract
In the cascade of the phenomena, leading to development of deregulation pathology, the important place is allocated to change of activity of brain structures, including reason of their immune damage, stimulation or blockade. Injection of antinigra antibodies to black substance is followed by decrease in efficiency of mechanisms of organ resistance in system of external breath that is shown in deterioration in biophysical properties of surfactant and reduction of number and activity of phagocyting alveolar macrophages.
Russian Journal of Immunology. 2017;20(3):531-533
531-533
INFLUENCE OF METHYLPREDNISOLON ON THE ACTIVATION OF TCR-STIMULATED CD4+CD45RO+ T-LYMPHOCYTES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN THE SYSTEM IN VITRO
Abstract
The effect of methylprednisolone (MP) on the functional activity of CD4+ CD45RO+ T cells obtained in patients with rheumatoid arthritis (RA) and healthy donors in the in vitro system was studied. It has been shown that MP significantly reduces the number of TCR-activated СD4+СD45RO+СD25+ and СD4+СD45RO+СD71+ T-lymphocytes in cultures of RA patients and in healthy donors. MP, reducing the number of СD4+СD28+ T cells, promotes the formation of terminal-differentiated memory effector T-cells.
Russian Journal of Immunology. 2017;20(3):534-536
534-536
CLINICAL AND IMMUNOLOGICAL EFFICIENCY OF BOVGUALURONIDAZA AZOKSIMER IN WOMEN WITH CHRONIC ENDOMETRITIS AND UTERINE FIBROIDS COMPLEX TREATMENT
Abstract
The aim of our study was to evaluate clinical and immunology efficiency of bovgualuronidaza azok-simer (“Longidaza”) in women with active nonspecific chronic endometritis and uterine fibroids treat-men. Bovgualuronidaza azoksimer inclusion in women with chronic endometritis of nonspecific etiology and uterine fibroids complex treatment increases clinical efficiency and improves indicators of innate immunity.
Russian Journal of Immunology. 2017;20(3):536-538
536-538
CONDITION OF CELLULAR AND HUMORAL LINKS OF IMMUNITY IN CHILDREN WITH COMMUNITY-ACQUIRED PNEUMONIA IN CHELYABINSK CITY
Abstract
In pediatric patients, pneumonia is one of the most urgent problems of modern medicine and the most important single cause of death in children worldwide. One of the leading causes of pneumonia at the present time is the change in the immunological reactivity of the organism. To identify features of the immune status, namely cellular and humoral links of immunity in children, we examined a total of 86 children aged 1 year to 18 years. All the children were divided into 2 groups: the first group of 60 children with a diagnosis of community-acquired pneumonia, the second group consisted of 26 healthy children of corresponding age and sex. Thus revealed the imbalance of cellular and humoral immunity in children with community-acquired pneumonia. Studies of the immune status in patients with pneumonia indicate a decrease in levels of serum immunoglobulins and phagocytosis, increased levels of circulating immune complexes.
Russian Journal of Immunology. 2017;20(3):538-540
538-540
INTERLEUKINE EARLY RISK MARKERS OF ACUTE REJECTION IN KIDNEY TRANSPLANTATION
Abstract
This article discusses the possibility of predicting post-transplant complications. The aim of our work is to evaluate the association of pre-operative level 21 of the cytokine in the blood plasma and during the post-transplant period. The analysis was first the differences in the initial level of biomarkers in patients without complications and with complications after transplantation. Our results confirmed that the screening of cytokines prior to transplantation may be used to predict the subsequent development of complications.
Russian Journal of Immunology. 2017;20(3):541-543
541-543
ASTHMA DIAGNOSIS IN PRESCHOOL CHILDREN
Abstract
Literature review shows, that no one of diagnostic method can be used for accurate asthma diagnosis in 5 years children from risk group of asthma development. Use of induced sputum helps us to reveal the chronic eosinophilic inflammation of airways mucosa and thus help us to diagnose asthma in children. Also induced sputum can help us to remove the asthma diagnosis.
Russian Journal of Immunology. 2017;20(3):543-545
543-545
STUDY OF MONONUCLEAR AND TUMOR CELLS MIGRATION TOWRDS TLRS LIGANDS AND CXCL12
Abstract
Chemotaxis and other factors of innate immunity are involved in metastasis (such as TLRs), it is advisable to study the effect of both TLRs and chemokines on the chemotaxis of tumor cells. The aim. Study and compare the chemotaxis of mononuclear cells and tumor cells towards TLRs ligands and CXCL12. Materials and methods. Chemoattractants were synthetic TLR ligands (DNA_lig, RNA_lig) and CXCL12. The study of cell migration was carried out in dynamics with the help of the Boyden chamber. Results. ^emotaxis of MNC from healthy donors exceeds the chemotaxis of tumor cells by 4 times and significantly differs by 1,6 times from spontaneous migration. Directional chemotaxis of MNCs of healthy donors in 1,7 and 1,5 times greater than the migration of MNCs of healthy donors after 60 minutes and 24 hours, respectively, relative to migration to DNA_lig and RNA_lig. Conclusion. From the results obtained, it can be seen that CXCL12-induced chemotaxis is higher in MNCs of healthy donors than in tumor cells. Similarly, chemotaxis relative to DNA_lig and RNA_lig is significantly lower than in the control. Thus, it can be said that TLRs ligands may inhibit migratory activity.
Russian Journal of Immunology. 2017;20(3):545-547
545-547
547-549
AUTOIMMUNE THROMBOCYTOPENY IN PREGNANT
Abstract
Simultaneous performance of laparoscopic splenectomy with elective caesarean section in pregnant women with severe refractory course of immune thrombocytopenia is a safe method, due to the low invasiveness, the expected lower blood loss and a fairly rapid postoperative recovery than is possible with an open access approach.
Russian Journal of Immunology. 2017;20(3):550-552
550-552
MILIACIN EFFECT ON MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS IN CASE OF EXPERIMENTAL SEPSIS
Abstract
Acute generalized infection caused by P aerugenosa in mice (CBAxC57Bl6)Fj, is characterized by more severe morphological and functional disorders than the same infection caused by K. pneumoniae. The use of miliacin has a protective effect in case of sepsis, makes structural disorders less severe and modulates functional activity of immune peritoneal macrophages.
Russian Journal of Immunology. 2017;20(3):552-554
552-554
DINAMYCS OF HUMORAL IMMUNITY INDICATORS IN PATIENTS WITH ASEPTIC NECROSIS OF THE FEMORAL HEAD
Abstract
The level of B-lymphocytes, the major classes of Ig, circulating immune complexes(CIC) in blood of patients with aseptic necrosis of the femoral head (ANFH) before and after(in a year) total joint replacement surgery has been investigated. A significant reduction of initially high levels of IgG, CIC and b-lymphocytes after joint replacement is determined. The levels of IgG in the blood of patients with ANFH are considered as markers of the effective treatment.
Russian Journal of Immunology. 2017;20(3):555-557
555-557
CYTOKINE PROFILE IN PATIENTS WITH ASEPTIC NECROSIS OF THE FEMORAL HEAD
Abstract
The levels of serum cytokines were investigated in 17 patients with aseptic necrosis of the femoral head before surgery total joint replacement aged between 33 and 57. A significant increase in the blood compared to normal levels of TNF-α, IL-17, IL - 4, IL-10 as well as the decrease of IFN-γ was determined. The identified imbalance of cytokines which are positively and negatively regulating the osteoclastogenesis may contribute to an increased resorption of bone tissue in this pathology.
Russian Journal of Immunology. 2017;20(3):557-559
557-559
THE ROLE OF CERTAIN BLOOD CYTOKINES AND FACTORS OF INNATE IMMUNITY FOR PREDICTION OF REACTIVE ARTHRITIS OUTCOME
Abstract
The clinical and immunological examination of children with reactive arthritis (ReA) and discriminant analysis allowed to establish significant serum factors associated with the clinical variant of ReA (IL-17, IL-6, INF-γ, lysozyme, CRP), which indicates a possible Use them as markers for predicting the clinical course of ReA and the outcome of arthritis.
Russian Journal of Immunology. 2017;20(3):559-561
559-561
INDICATORS OF THE LEUKOCYTAL PART OF THE IMMUNE SYSTEM IN MATURE AGE WOMEN IN DYNAMIC OF REHABILITATION AFTER THE UPPER LIMB INJURY
Abstract
The article presents the results of the study of the leukocyte counts in the blood of mature age women in dynamic of rehabilitation period after the Colles fracture. The first 3-7 days of the recovery period after the immobilizing dressing removal were accompanied by a significant stress response for the organism of women of the studuied groups. The marked leukocytosis, neutrophilia, monocytosis and lymphopenia were observed in the peripheral blood of women during this period. The stress response to the rehabilitation activities in the women of the main group was less than in the control group, which proves the effectiveness of the proposed complex of auto relaxing exercises.
Russian Journal of Immunology. 2017;20(3):562-564
562-564
FEATURES OF CONGENITAL IMMUNITY OF PREMATURE INFANTS WITH SEVERE HYPOXIC-ISCHEMIC LESIONS OF THE CENTRAL NERVOUS SYSTEM
Abstract
With the purpose of revealing the features of the innate immunity of premature infants with severe hypoxic-ischemic injury of the central nervous system, depending on the gestational age, a survey of deeply preterm infants born at the time of very early and early premature births was conducted. It was established that in all premature infants with severe hypoxic-ischemic CNS lesions, the level of expression of adhesion molecules on monocytes (CD14+CD11b+) and an increase in the level of IFN-γ and IL-8 at birth was observed at birth, regardless of the gestational age. The peculiarity of the immune response of children born in the term of early premature births is a decrease in the relative number of activated monocytes of CD14+CD64+ and CD14+HLA-DR, which indicates a disruption in the relationship between the cells of congenital and adaptive immunity and may be the cause of bacterial complications and a prolonged course of inflammatory Process.
Russian Journal of Immunology. 2017;20(3):564-566
564-566
FEATURES OF LUMINAL-DEPENDENT CHEMILUMINESCENCE REACTION UNER THE COLD STRESS. OPIATE MECHANISMS
Abstract
Acute cold stress (4°C, 4 hours) led to inhibition of the production of ROS (reactive oxygen species) in stimulated cultures of peritoneal macrophages of the mouse. The effect of acute cold stress was not modified by the administration of naloxone. Chronic cold stress (4°C, 4 hours, 7 days) led to stimulation of ROS production by peritoneal macrophages of mice in the presence of zymosan. This effect is partially canceled by the administration of naloxone.
Russian Journal of Immunology. 2017;20(3):566-568
566-568
568-570
EFFECT OF DEHYDROEPIANDROSTERONE ON THE LUMINOL- DEPENDENT CHEMILUMINESCENCE RESPONSE UNDER ZYMOSAN-INDUCED PERITONITIS IN OLD RATS
Abstract
It was established that level of the luminol-dependent chemiluminescence reaction in old male rats under zymosan-induced peritonitis was higher in comparison with young rats. The introduction of dehy-droepiandrosterone to the old rats results in a marked decrease in the luminol-dependent chemiluminescence with peritoneal cells.
Russian Journal of Immunology. 2017;20(3):570-572
570-572
REGULATION OF NK CELLS FUNCTIONAL ACTIVITY BY REPRODUCTIVE HORMONES
Abstract
The influence of reproductive hormones on phenotypic characteristics, cytokine production and the level of lytic activity of natural killer (NK) cells was studied. The role of miRNAs in the hormonal mechanisms of formation of regulatory and cytotoxic NK cells subpopulations during pregnancy was investigated. The new physiological mechanisms of endocrine control of mother’s immune system NK cells that can be implemented in different periods of pregnancy were identified as a result of this work.
Russian Journal of Immunology. 2017;20(3):572-574
572-574
DETERMINE THE EFFECT OF NEUTROPHILS SECRETORY PRODUCTS, PRE-EXPOSED SIMULATE LOW-INTENSITY ELECTROMAGNETIC RADIATION ON BIOFILM FORMATION
Abstract
The present study analyzed the influence of the simulated electromagnetic radiation at microwave anti-biofilm-forming neutrophil secretory activity products. It is established that electromagnetic radiation, both technogenic and natural, affects the functioning of cells of innate immunity. This information opens up prospects for correcting dysbiosis.
Russian Journal of Immunology. 2017;20(3):574-576
574-576
THE STUDY OF EFFECT TO USE THE PLATELET RICH PLASMA FOR IMMUNE STATUS OF THE ORAL CAVITY TO PATIENTS WITH NON- REMOVABLE DENTURES
Abstract
The aim of this study was to study the effect of the injectable form of platelet rich autoplasma on immunological indices of the oral cavity to fixed prostheses patients. The number and viability of leukocytes and phagocytic function of neutrophils of oral fluid to fixed prostheses patients was significantly lower than to patients without dentures. However, after the injection of platelet rich autoplasma indicators of cellular immunity to patients with fixed prostheses approached that of patients without dentures, which indicates a positive immunological effects of injection of platelet rich autoplasma.
Russian Journal of Immunology. 2017;20(3):577-579
577-579
HEPATITIS C VIRUS COINFECTION DISTURBS NAIVE CD4+ T-CELL HOMEOSTASIS IN HIV-INFECTED PATIENTS
Abstract
It has been established that during HIV/HCV coinfection a naive СD4+ T-cell deficiency and a disruption of the cell transition between the two subsets: СD4+ recent thymic migrants and the mature СD4+ naive T cells, is developed. In HIV/HCV coinfection, unlike HIV monoinfection, the dependence of homeostatic proliferation on T-cell count is disturbed.
Russian Journal of Immunology. 2017;20(3):579-581
579-581
DEVELOPMENT OF A MEDICINAL PRODUCT ON THE BASIS OF INTERFERON ALPHA IN COMBINATION WITH ANTIOXIDANTS, WHICH HAS IMMUNOMODELLING ACTIVITY
Abstract
The purpose of the study was the choice of composition, development of technology and the study of immunomodelling activity of suppositories, containing interferon alpha in combination acides nicotnic and succinic. When choosing the composition hydrophilic diphilic and lipophilic bases were examined. It was established that the dosage forms prepared with the use of polyethylene glycols provided a uniform plank mass. Taking into account the process of liberation in the conditions of dissolution temperature as well as spectrophotometric determination, the technology of the novel form of the drug form - suppositories with interferon alpha in combination with antioxidants.
Russian Journal of Immunology. 2017;20(3):581-583
581-583