INVESTIGATION OF BIOSPECIFIC INTERACTION BETWEEN DNA-APTAMER AND CARBON NANOPARTICLES

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Abstract

Aptamers are DNA- and RNA-oligonucleotides that can selectively bind target molecules. Objective of our work was to investigate into interaction of biotinylated DNA-aptamer specific to human IgE with carbon nanoparticles covalently conjugated with streptavidin. Maximal level of specific interaction (about 40 pM per 1 mg of nanoparticles) was observed when concentration of aptamer was 100 nM.

About the authors

M. BA Rayev

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

P. V.1· Khramtsov

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

M. S. Bochkova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

V. P. Timganova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

S. A.1· Zamorina

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

M. D. Kropaneva

Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2017 Rayev M.B., Khramtsov P.V., Bochkova M.S., Timganova V.P., Zamorina S.A., Kropaneva M.D.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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