INVESTIGATION OF GROWTH AND VIABILITY OF HYBRIDOMA BAP3 CELLS IN DIFFERENT CULTURE MEDIA IN VITRO

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Abstract

Growth and viability in different culture media after thawing of hybridoma BAP3 cells that produces monoclonal antibodies against pregnancy-specific b1-glycoprotein (PSG) were studied in vitro. We show that cultivation in [DMEM+12 % FCS] leads to maximal cell growth (1,8-fold in two days) with retention of viability of more than 70 %. There were 1,1-1,2-fold growth and viability of 55-59 % in [DCCM-1]-based media and 1,6-fold growth in two days in [RPMI-1640+12 % FCS]. Therefore, application of [DMEM+12 % FCS] medium seems to be the most appropriate for cultivation of hybridoma BAP3 cells after their thawing from cryopreservation.

About the authors

M. B. Raev

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Author for correspondence.
Russian Federation

S. A. Zamorina

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Russian Federation

V. P. Timganova

Institute of Ecology and Genetics of Microorganisms UB RAS

Russian Federation

M. S. Bochkova

Institute of Ecology and Genetics of Microorganisms UB RAS

Russian Federation

P. V. Khramtsov

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Russian Federation

References

  1. Раев М. Б. Способ выделения и очистки трофо-бластического β-1-гликопротеина. Патент РФ № 2367449, опубликован 20.09.2009, Бюл. № 26.

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Copyright (c) 2017 Raev M.B., Zamorina S.A., Timganova V.P., Bochkova M.S., Khramtsov P.V.

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