CNT-Based Label-Free Electrochemical Sensing of Native DNA with Allele Single Nucleotide Polymorphism
- Autores: Grushevskaya H.1, Krylova N.1, Lipnevich I.1, Babenka A.2, Egorova V.3, Chakukov R.1
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Afiliações:
- Belarusian State University
- Belarusian State Medical University
- Belarusian State Pedagogical University
- Edição: Volume 52, Nº 14 (2018)
- Páginas: 1836-1838
- Seção: Nanostructure Devices
- URL: https://journals.rcsi.science/1063-7826/article/view/205036
- DOI: https://doi.org/10.1134/S1063782618140117
- ID: 205036
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Resumo
A label-free high-performance electrochemical sensing of single nucleotide polymorphism in native genomic DNA samples from cancer tissues, in oncogene-specific complementary DNA and in amplicons has been carried out on bundles of few-walled carboxylated carbon nanotubes, surface of which was coated with electron-dense layer of probe DNA. High selectivity and sensitivity of the novel DNA-nanosensor are ensured by the following features. Firstly, a complementary-hybridization signal is enhanced by resonance between plasmons in carbon nanotubes and dipole oscillations of hydrated nucleic acids located in near-electrode Helmholtz double layer of the impedance sensor. Secondly, a decoration of carbon nanotubes by adhered metal atoms in a high-spin state leads to Fermi level stabilization for the carbon nanotube bundles.
Sobre autores
H. Grushevskaya
Belarusian State University
Autor responsável pela correspondência
Email: grushevskaja@bsu.by
Belarus, Minsk
N. Krylova
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk
I. Lipnevich
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk
A. Babenka
Belarusian State Medical University
Email: grushevskaja@bsu.by
Belarus, Minsk
V. Egorova
Belarusian State Pedagogical University
Email: grushevskaja@bsu.by
Belarus, Minsk
R. Chakukov
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk