CNT-Based Label-Free Electrochemical Sensing of Native DNA with Allele Single Nucleotide Polymorphism
- Authors: Grushevskaya H.V.1, Krylova N.G.1, Lipnevich I.V.1, Babenka A.S.2, Egorova V.P.3, Chakukov R.F.1
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Affiliations:
- Belarusian State University
- Belarusian State Medical University
- Belarusian State Pedagogical University
- Issue: Vol 52, No 14 (2018)
- Pages: 1836-1838
- Section: Nanostructure Devices
- URL: https://journals.rcsi.science/1063-7826/article/view/205036
- DOI: https://doi.org/10.1134/S1063782618140117
- ID: 205036
Cite item
Abstract
A label-free high-performance electrochemical sensing of single nucleotide polymorphism in native genomic DNA samples from cancer tissues, in oncogene-specific complementary DNA and in amplicons has been carried out on bundles of few-walled carboxylated carbon nanotubes, surface of which was coated with electron-dense layer of probe DNA. High selectivity and sensitivity of the novel DNA-nanosensor are ensured by the following features. Firstly, a complementary-hybridization signal is enhanced by resonance between plasmons in carbon nanotubes and dipole oscillations of hydrated nucleic acids located in near-electrode Helmholtz double layer of the impedance sensor. Secondly, a decoration of carbon nanotubes by adhered metal atoms in a high-spin state leads to Fermi level stabilization for the carbon nanotube bundles.
About the authors
H. V. Grushevskaya
Belarusian State University
Author for correspondence.
Email: grushevskaja@bsu.by
Belarus, Minsk
N. G. Krylova
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk
I. V. Lipnevich
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk
A. S. Babenka
Belarusian State Medical University
Email: grushevskaja@bsu.by
Belarus, Minsk
V. P. Egorova
Belarusian State Pedagogical University
Email: grushevskaja@bsu.by
Belarus, Minsk
R. F. Chakukov
Belarusian State University
Email: grushevskaja@bsu.by
Belarus, Minsk