Synthesis of the Tripeptides Tyr-Thr-Lys Phosphorylated with Isopropyl Methyl- and (Deuteromethyl)phosphonochloridates as Reference Standards for the Analysis of Biomedical Samples
- Authors: Rodin I.A.1, Baygildiev T.M.1, Krylov V.I.2, Osipov V.N.3, Krylov I.I.2, Yashkir V.A.2, Rybalchenko I.V.2
-
Affiliations:
- Chemical Department
- 27 Research Center
- Farmsintez ZAO
- Issue: Vol 89, No 10 (2019)
- Pages: 2103-2107
- Section: Article
- URL: https://journals.rcsi.science/1070-3632/article/view/223124
- DOI: https://doi.org/10.1134/S1070363219100189
- ID: 223124
Cite item
Abstract
A procedure for the phosphorylation of the tripeptide Tyr-Thr-Lys with isopropyl methyl- or (deuteromethyl)phosphonochloridate is developed. The phosphorylated tripeptides are intended for use as reference standards in the analysis of blood samples of people suspected to have been exposed to acetylcholinesterase inhibitors. Conditions of hromatographic separation and purification of the synthesized compounds are determined and optimized, which ensures the preparation of high-purity phosphorylated tripeptides.
Keywords
About the authors
I. A. Rodin
Chemical Department
Author for correspondence.
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 119991
T. M. Baygildiev
Chemical Department
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 119991
V. I. Krylov
27 Research Center
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 105005
V. N. Osipov
Farmsintez ZAO
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 111024
I. I. Krylov
27 Research Center
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 105005
V. A. Yashkir
27 Research Center
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 105005
I. V. Rybalchenko
27 Research Center
Email: igorrodin@yandex.ru
Russian Federation, Moscow, 105005
![](/img/style/loading.gif)