Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence
- Авторы: Gorshkov A.1, Pridatchenko M.2, Perlova T.2, Tarasova I.2, Levitsky L.2, Gorshkov M.2, Evreinov V.1
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Учреждения:
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Tal’rose Institute for Energy Problems of Chemical Physics
- Выпуск: Том 71, № 12 (2016)
- Страницы: 1188-1197
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182195
- DOI: https://doi.org/10.1134/S106193481610004X
- ID: 182195
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Аннотация
The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.
Об авторах
A. Gorshkov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119991
M. Pridatchenko
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119334
T. Perlova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119334
I. Tarasova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119334
L. Levitsky
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119334
M. Gorshkov
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119334
V. Evreinov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: evreinov@polymer.chph.ras.ru
Россия, Moscow, 119991