Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence

A. V. Gorshkov; M. L. Pridatchenko; T. Yu. Perlova; I. A. Tarasova; L. I. Levitsky; M. V. Gorshkov; V. V. Evreinov

doi:10.1134/S106193481610004X

Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence

Authors: Gorshkov A.V.¹, Pridatchenko M.L.², Perlova T.Y.², Tarasova I.A.², Levitsky L.I.², Gorshkov M.V.², Evreinov V.V.¹
Affiliations:
1. Semenov Institute of Chemical Physics, Russian Academy of Sciences
2. Tal’rose Institute for Energy Problems of Chemical Physics
Issue: Vol 71, No 12 (2016)
Pages: 1188-1197
Section: Articles
URL: https://journals.rcsi.science/1061-9348/article/view/182195
DOI: https://doi.org/10.1134/S106193481610004X
ID: 182195

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Abstract

The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.