Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence
- Autores: Gorshkov A.1, Pridatchenko M.2, Perlova T.2, Tarasova I.2, Levitsky L.2, Gorshkov M.2, Evreinov V.1
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Afiliações:
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Tal’rose Institute for Energy Problems of Chemical Physics
- Edição: Volume 71, Nº 12 (2016)
- Páginas: 1188-1197
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182195
- DOI: https://doi.org/10.1134/S106193481610004X
- ID: 182195
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Resumo
The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.
Sobre autores
A. Gorshkov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119991
M. Pridatchenko
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119334
T. Perlova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119334
I. Tarasova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119334
L. Levitsky
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119334
M. Gorshkov
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119334
V. Evreinov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Autor responsável pela correspondência
Email: evreinov@polymer.chph.ras.ru
Rússia, Moscow, 119991
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