Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence
- Authors: Gorshkov A.V.1, Pridatchenko M.L.2, Perlova T.Y.2, Tarasova I.A.2, Levitsky L.I.2, Gorshkov M.V.2, Evreinov V.V.1
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Affiliations:
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Tal’rose Institute for Energy Problems of Chemical Physics
- Issue: Vol 71, No 12 (2016)
- Pages: 1188-1197
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182195
- DOI: https://doi.org/10.1134/S106193481610004X
- ID: 182195
Cite item
Abstract
The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.
About the authors
A. V. Gorshkov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119991
M. L. Pridatchenko
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119334
T. Yu. Perlova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119334
I. A. Tarasova
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119334
L. I. Levitsky
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119334
M. V. Gorshkov
Tal’rose Institute for Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119334
V. V. Evreinov
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Author for correspondence.
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Moscow, 119991