电邮这篇文章 Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
- 作者: Romanov V.P.1, Kostromina T.I.2, Miroshnikov A.I.2, Feofanov S.A.1
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隶属关系:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- 期: 卷 42, 编号 6 (2016)
- 页面: 631-637
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228196
- DOI: https://doi.org/10.1134/S1068162016040154
- ID: 228196
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详细
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.
作者简介
V. Romanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290
T. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
俄罗斯联邦, Moscow, 117997
A. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
俄罗斯联邦, Moscow, 117997
S. Feofanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: feo10@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290
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