Отправить статью по E-mail Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
- Авторы: Romanov V.P.1, Kostromina T.I.2, Miroshnikov A.I.2, Feofanov S.A.1
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Учреждения:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Выпуск: Том 42, № 6 (2016)
- Страницы: 631-637
- Раздел: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228196
- DOI: https://doi.org/10.1134/S1068162016040154
- ID: 228196
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Аннотация
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.
Об авторах
V. Romanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Россия, Pushchino, Moscow oblast, 142290
T. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Россия, Moscow, 117997
A. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Россия, Moscow, 117997
S. Feofanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Автор, ответственный за переписку.
Email: feo10@mail.ru
Россия, Pushchino, Moscow oblast, 142290
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