Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli


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Аннотация

A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.

Авторлар туралы

V. Romanov

Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: feo10@mail.ru
Ресей, Pushchino, Moscow oblast, 142290

T. Kostromina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: feo10@mail.ru
Ресей, Moscow, 117997

A. Miroshnikov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: feo10@mail.ru
Ресей, Moscow, 117997

S. Feofanov

Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Хат алмасуға жауапты Автор.
Email: feo10@mail.ru
Ресей, Pushchino, Moscow oblast, 142290

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