Enviar artigo por via de e-mail Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
- Autores: Romanov V.P.1, Kostromina T.I.2, Miroshnikov A.I.2, Feofanov S.A.1
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Afiliações:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Edição: Volume 42, Nº 6 (2016)
- Páginas: 631-637
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228196
- DOI: https://doi.org/10.1134/S1068162016040154
- ID: 228196
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Resumo
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.
Sobre autores
V. Romanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Rússia, Pushchino, Moscow oblast, 142290
T. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Rússia, Moscow, 117997
A. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Rússia, Moscow, 117997
S. Feofanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Autor responsável pela correspondência
Email: feo10@mail.ru
Rússia, Pushchino, Moscow oblast, 142290
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