Мақаланы E-mail арқылы жіберу Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
- Авторлар: Romanov V.P.1, Kostromina T.I.2, Miroshnikov A.I.2, Feofanov S.A.1
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Мекемелер:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Шығарылым: Том 42, № 6 (2016)
- Беттер: 631-637
- Бөлім: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228196
- DOI: https://doi.org/10.1134/S1068162016040154
- ID: 228196
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Аннотация
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.
Авторлар туралы
V. Romanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Ресей, Pushchino, Moscow oblast, 142290
T. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Ресей, Moscow, 117997
A. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Ресей, Moscow, 117997
S. Feofanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Хат алмасуға жауапты Автор.
Email: feo10@mail.ru
Ресей, Pushchino, Moscow oblast, 142290
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