Email this article Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
- Authors: Romanov V.P.1, Kostromina T.I.2, Miroshnikov A.I.2, Feofanov S.A.1
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Affiliations:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 42, No 6 (2016)
- Pages: 631-637
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228196
- DOI: https://doi.org/10.1134/S1068162016040154
- ID: 228196
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Abstract
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.
About the authors
V. P. Romanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290
T. I. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Russian Federation, Moscow, 117997
A. I. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: feo10@mail.ru
Russian Federation, Moscow, 117997
S. A. Feofanov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: feo10@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290
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