SELECTION OF AN AMINO ACID SITE WITH ONE OF THE FASTEST CLEAVAGE KINETICS BY THE ENDOSOMAL PROTEASE CATHEPSIN B FOR POTENTIAL USE IN DRUG DELIVERY SYSTEMS
- 作者: Khramtsov Y.1, Georgiev G.1, Sobolev A.1,2
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隶属关系:
- Institute of Gene Biology, RAS
- Lomonosov Moscow State University
- 期: 卷 509, 编号 1 (2023)
- 页面: 211-214
- 栏目: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/135675
- DOI: https://doi.org/10.31857/S2686738923700166
- EDN: https://elibrary.ru/NBOJNC
- ID: 135675
如何引用文章
详细
Based on known literature data, six peptide sequences were selected that are potentially capable of being rapidly cleaved by the endosomal protease cathepsin B. For comparison, cathepsin B cleavage of common linker sequences, polyglycine and polyglycine-serine, was also studied. Different ends of these peptides were labeled with sulfoCyanine3 and sulfoCyanine5 fluorescent dyes, between which Förster resonant energy transfer (FRET) is possible. The kinetics of cleavage of peptides by cathepsin B was studied on a multimodal plate reader by FRET signal reduction. FKFL and FRRG cleavage sites have been shown to be the most suitable for potential use in various drug delivery systems. These sites are much more efficiently cleaved under slightly acidic conditions of endosomes than at neutral extracellular pH.
作者简介
Y. Khramtsov
Institute of Gene Biology, RAS
编辑信件的主要联系方式.
Email: ykhram2000@mail.ru
Russian, Moscow
G. Georgiev
Institute of Gene Biology, RAS
Email: alsobolev@yandex.ru
Russian, Moscow
A. Sobolev
Institute of Gene Biology, RAS; Lomonosov Moscow State University
编辑信件的主要联系方式.
Email: alsobolev@yandex.ru
Russian, Moscow; Russian, Moscow
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