Preparation of temozolomide-loaded polymer particles and study of their antitumor activity in models of glioma and melanoma
- Authors: Nikolskaya E.D.1, Zhunina O.A.1, Vasilenko E.A.2, Yabbarov N.G.1, Tereshchenko O.G.1, Sokol M.B.1, Popenko V.I.3, Severin E.S.1
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Affiliations:
- Russian Research Center for Molecular Diagnostics and Therapy
- Nizhny Novgorod State University
- Engelhardt Institute of Molecular Biology
- Issue: Vol 43, No 5 (2017)
- Pages: 552-560
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228665
- DOI: https://doi.org/10.1134/S1068162017050090
- ID: 228665
Cite item
Abstract
Temozolomide (TMZ) is a cytostatic drug used in treatment of patients with malignant gliomas and melanomas. The development of innovative formulations for delivery of TMZ into target cells in order to reduce its systemic toxicity and gain prolonged effect is a topical problem of modern biotechnology and pharmacology. The article presents experimental data on development of the TMZ polymer-based formulation. The TMZ formulation presents nanoparticles with average size of 300 nm exhibiting high in vitro cytotoxic activity against melanoma and glioma cells (cell lines C6, U377MG, B16, and Mel-10). Melanoma (B16 cell line) bearing mice (C57Bl/61) treated by TMZ polymer nanoparticles revealed inhibition of tumor growth and increase of the animal lifespan. Also, loading of TMZ into the polymer particles was shown to decrease acute toxicity of the drug compared to the intact drug.
Keywords
About the authors
E. D. Nikolskaya
Russian Research Center for Molecular Diagnostics and Therapy
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149
O. A. Zhunina
Russian Research Center for Molecular Diagnostics and Therapy
Author for correspondence.
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149
E. A. Vasilenko
Nizhny Novgorod State University
Email: olga_yarova@bk.ru
Russian Federation, Nizhny Novgorod, 603950
N. G. Yabbarov
Russian Research Center for Molecular Diagnostics and Therapy
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149
O. G. Tereshchenko
Russian Research Center for Molecular Diagnostics and Therapy
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149
M. B. Sokol
Russian Research Center for Molecular Diagnostics and Therapy
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149
V. I. Popenko
Engelhardt Institute of Molecular Biology
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 119991
E. S. Severin
Russian Research Center for Molecular Diagnostics and Therapy
Email: olga_yarova@bk.ru
Russian Federation, Moscow, 117149