Synthesis of Random DNA Libraries for In Vitro Selection and Analysis of Their Nucleotide Composition
- Authors: Davydova A.S.1, Krasheninina O.A.1, Tupikin A.E.1, Kabilov M.R.1, Venyaminova A.G.1, Vorobyeva M.A.1
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine SB RAS
- Issue: Vol 45, No 6 (2019)
- Pages: 656-661
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229285
- DOI: https://doi.org/10.1134/S1068162019060141
- ID: 229285
Cite item
Abstract
Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers with specified properties could be obtained from combinatorial libraries of nucleic acids by in vitro selection procedure. Here, we optimized conditions of chemical synthesis of DNA library on the automated ASM-800 DNA/RNA synthesizer. Enzymatic hydrolysis and high-throughput sequencing methods were used to analyze compositions of synthesized combinatorial libraries. The DNA library generated under the chosen conditions is characterized by the sufficiently uniform randomization and can be applied for the in vitro selection.
About the authors
A. S. Davydova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Author for correspondence.
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
O. A. Krasheninina
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
A. E. Tupikin
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
M. R. Kabilov
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
A. G. Venyaminova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
M. A. Vorobyeva
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090