Synthesis of Random DNA Libraries for In Vitro Selection and Analysis of Their Nucleotide Composition
- 作者: Davydova A.1, Krasheninina O.1, Tupikin A.1, Kabilov M.1, Venyaminova A.1, Vorobyeva M.1
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine SB RAS
- 期: 卷 45, 编号 6 (2019)
- 页面: 656-661
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229285
- DOI: https://doi.org/10.1134/S1068162019060141
- ID: 229285
如何引用文章
详细
Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers with specified properties could be obtained from combinatorial libraries of nucleic acids by in vitro selection procedure. Here, we optimized conditions of chemical synthesis of DNA library on the automated ASM-800 DNA/RNA synthesizer. Enzymatic hydrolysis and high-throughput sequencing methods were used to analyze compositions of synthesized combinatorial libraries. The DNA library generated under the chosen conditions is characterized by the sufficiently uniform randomization and can be applied for the in vitro selection.
作者简介
A. Davydova
Institute of Chemical Biology and Fundamental Medicine SB RAS
编辑信件的主要联系方式.
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
O. Krasheninina
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Tupikin
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
M. Kabilov
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Venyaminova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
M. Vorobyeva
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090