Synthesis of Random DNA Libraries for In Vitro Selection and Analysis of Their Nucleotide Composition
- Авторы: Davydova A.1, Krasheninina O.1, Tupikin A.1, Kabilov M.1, Venyaminova A.1, Vorobyeva M.1
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Учреждения:
- Institute of Chemical Biology and Fundamental Medicine SB RAS
- Выпуск: Том 45, № 6 (2019)
- Страницы: 656-661
- Раздел: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229285
- DOI: https://doi.org/10.1134/S1068162019060141
- ID: 229285
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Аннотация
Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers with specified properties could be obtained from combinatorial libraries of nucleic acids by in vitro selection procedure. Here, we optimized conditions of chemical synthesis of DNA library on the automated ASM-800 DNA/RNA synthesizer. Enzymatic hydrolysis and high-throughput sequencing methods were used to analyze compositions of synthesized combinatorial libraries. The DNA library generated under the chosen conditions is characterized by the sufficiently uniform randomization and can be applied for the in vitro selection.
Об авторах
A. Davydova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Автор, ответственный за переписку.
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090
O. Krasheninina
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090
A. Tupikin
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090
M. Kabilov
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090
A. Venyaminova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090
M. Vorobyeva
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: anna.davydova@niboch.nsc.ru
Россия, Novosibirsk, 630090