Email this article Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens
- Authors: Krylov V.B.1, Petruk M.I.1, Grigoryev I.V.2, Lebedin Y.S.2, Glushko N.I.3, Khaldeeva E.V.3, Argunov D.A.1, Khatuntseva E.A.1, Toplishek M.V.1, Komarova B.S.1, Karelin A.A.1, Yudina O.N.1, Menshov V.M.1, Yashunskii D.V.1, Tsvetkov Y.E.1, Nifantiev N.E.1
-
Affiliations:
- Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
- XEMA Company Limited
- Kazan Scientific Research Institute of Epidemiology and Microbiology
- Issue: Vol 44, No 1 (2018)
- Pages: 80-89
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228804
- DOI: https://doi.org/10.1134/S1068162017060073
- ID: 228804
Cite item
Open Access
Access granted
Subscription Access
Abstract
The carbohydrate specificity of antibodies obtained by immunizing laboratory animals with immunogens prepared from the Aspergillus fumigatus cell wall was analyzed using the library of biotinylated synthetic oligosaccharides. It has been shown that the main part of antipolysaccharide antibodies recognizes galactomannan in the studied immunogens with preference to its fragments containing more than two β-(1→5)-linked galactofuranosyl units. The data obtained can form the basis for the development of enzyme immunoassay for the detection of this dangerous fungal pathogen.
Keywords
About the authors
V. B. Krylov
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
M. I. Petruk
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
I. V. Grigoryev
XEMA Company Limited
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 105264
Y. S. Lebedin
XEMA Company Limited
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 105264
N. I. Glushko
Kazan Scientific Research Institute of Epidemiology and Microbiology
Email: nen@ioc.ac.ru
Russian Federation, Kazan, 420015
E. V. Khaldeeva
Kazan Scientific Research Institute of Epidemiology and Microbiology
Email: nen@ioc.ac.ru
Russian Federation, Kazan, 420015
D. A. Argunov
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
E. A. Khatuntseva
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
M. V. Toplishek
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
B. S. Komarova
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
A. A. Karelin
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
O. N. Yudina
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
V. M. Menshov
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
D. V. Yashunskii
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
Y. E. Tsvetkov
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
N. E. Nifantiev
Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry
Author for correspondence.
Email: nen@ioc.ac.ru
Russian Federation, Moscow, 119991
Supplementary files
