Email this article Investigation of the complex antibiotic INA-5812
- Authors: Lapchinskaya O.A.1, Katrukha G.S.1, Gladkikh E.G.1, Kulyaeva V.V.1, Coodan P.V.2, Topolyan A.P.3, Alferova V.A.1, Pogozheva V.V.1, Sukonnikov M.A.4, Rogozhin E.A.1,3, Prokhorenko I.A.1,3, Brylev V.A.3, Korolev A.M.1, Slyundina M.S.1,5, Borisov R.S.5,6, Serebryakova M.V.7, Shuvalov M.V.1,4, Ksenofontov A.L.7, Stoyanova L.G.8, Osterman I.A.4,7, Formanovsky A.A.3, Tashlitsky V.N.4, Baratova L.A.7, Timofeeva A.V.7, Tyurin A.P.1,3
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Affiliations:
- Gause Institute of New Antibiotics
- Federal Research Center of Nutrition and Biotechnology
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Chemistry
- Topchiev Institute of Petrochemical Synthesis
- Faculty of Physics, Mathematics and Natural Sciences
- Belozersky Institute of Physico-Chemical Biology
- Faculty of Biology
- Issue: Vol 42, No 6 (2016)
- Pages: 664-671
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228225
- DOI: https://doi.org/10.1134/S1068162016060078
- ID: 228225
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Abstract
A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.
About the authors
O. A. Lapchinskaya
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
G. S. Katrukha
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
E. G. Gladkikh
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
V. V. Kulyaeva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
P. V. Coodan
Federal Research Center of Nutrition and Biotechnology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 109240
A. P. Topolyan
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 117997
V. A. Alferova
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
V. V. Pogozheva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
M. A. Sukonnikov
Faculty of Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119991
E. A. Rogozhin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021; Moscow, 117997
I. A. Prokhorenko
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021; Moscow, 117997
V. A. Brylev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 117997
A. M. Korolev
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021
M. S. Slyundina
Gause Institute of New Antibiotics; Topchiev Institute of Petrochemical Synthesis
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021; Moscow, 119991
R. S. Borisov
Topchiev Institute of Petrochemical Synthesis; Faculty of Physics, Mathematics and Natural Sciences
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119991; Moscow, 115419
M. V. Serebryakova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119992
M. V. Shuvalov
Gause Institute of New Antibiotics; Faculty of Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021; Moscow, 119991
A. L. Ksenofontov
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119992
L. G. Stoyanova
Faculty of Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119991
I. A. Osterman
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119991; Moscow, 119992
A. A. Formanovsky
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 117997
V. N. Tashlitsky
Faculty of Chemistry
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119991
L. A. Baratova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119992
A. V. Timofeeva
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119992
A. P. Tyurin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: ap2rin@gmail.com
Russian Federation, Moscow, 119021; Moscow, 117997
