Investigation of the complex antibiotic INA-5812
- 作者: Lapchinskaya O.1, Katrukha G.1, Gladkikh E.1, Kulyaeva V.1, Coodan P.2, Topolyan A.3, Alferova V.1, Pogozheva V.1, Sukonnikov M.4, Rogozhin E.1,3, Prokhorenko I.1,3, Brylev V.3, Korolev A.1, Slyundina M.1,5, Borisov R.5,6, Serebryakova M.7, Shuvalov M.1,4, Ksenofontov A.7, Stoyanova L.8, Osterman I.4,7, Formanovsky A.3, Tashlitsky V.4, Baratova L.7, Timofeeva A.7, Tyurin A.1,3
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隶属关系:
- Gause Institute of New Antibiotics
- Federal Research Center of Nutrition and Biotechnology
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Chemistry
- Topchiev Institute of Petrochemical Synthesis
- Faculty of Physics, Mathematics and Natural Sciences
- Belozersky Institute of Physico-Chemical Biology
- Faculty of Biology
- 期: 卷 42, 编号 6 (2016)
- 页面: 664-671
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228225
- DOI: https://doi.org/10.1134/S1068162016060078
- ID: 228225
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详细
A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.
作者简介
O. Lapchinskaya
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
G. Katrukha
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
E. Gladkikh
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
V. Kulyaeva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
P. Coodan
Federal Research Center of Nutrition and Biotechnology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 109240
A. Topolyan
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 117997
V. Alferova
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
V. Pogozheva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
M. Sukonnikov
Faculty of Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119991
E. Rogozhin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021; Moscow, 117997
I. Prokhorenko
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021; Moscow, 117997
V. Brylev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 117997
A. Korolev
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021
M. Slyundina
Gause Institute of New Antibiotics; Topchiev Institute of Petrochemical Synthesis
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021; Moscow, 119991
R. Borisov
Topchiev Institute of Petrochemical Synthesis; Faculty of Physics, Mathematics and Natural Sciences
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119991; Moscow, 115419
M. Serebryakova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119992
M. Shuvalov
Gause Institute of New Antibiotics; Faculty of Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021; Moscow, 119991
A. Ksenofontov
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119992
L. Stoyanova
Faculty of Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119991
I. Osterman
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119991; Moscow, 119992
A. Formanovsky
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 117997
V. Tashlitsky
Faculty of Chemistry
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119991
L. Baratova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119992
A. Timofeeva
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119992
A. Tyurin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: ap2rin@gmail.com
俄罗斯联邦, Moscow, 119021; Moscow, 117997