Enviar artigo por via de e-mail Investigation of the complex antibiotic INA-5812
- Autores: Lapchinskaya O.1, Katrukha G.1, Gladkikh E.1, Kulyaeva V.1, Coodan P.2, Topolyan A.3, Alferova V.1, Pogozheva V.1, Sukonnikov M.4, Rogozhin E.1,3, Prokhorenko I.1,3, Brylev V.3, Korolev A.1, Slyundina M.1,5, Borisov R.5,6, Serebryakova M.7, Shuvalov M.1,4, Ksenofontov A.7, Stoyanova L.8, Osterman I.4,7, Formanovsky A.3, Tashlitsky V.4, Baratova L.7, Timofeeva A.7, Tyurin A.1,3
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Afiliações:
- Gause Institute of New Antibiotics
- Federal Research Center of Nutrition and Biotechnology
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Chemistry
- Topchiev Institute of Petrochemical Synthesis
- Faculty of Physics, Mathematics and Natural Sciences
- Belozersky Institute of Physico-Chemical Biology
- Faculty of Biology
- Edição: Volume 42, Nº 6 (2016)
- Páginas: 664-671
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228225
- DOI: https://doi.org/10.1134/S1068162016060078
- ID: 228225
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Resumo
A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.
Sobre autores
O. Lapchinskaya
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
G. Katrukha
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
E. Gladkikh
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
V. Kulyaeva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
P. Coodan
Federal Research Center of Nutrition and Biotechnology
Email: ap2rin@gmail.com
Rússia, Moscow, 109240
A. Topolyan
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 117997
V. Alferova
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
V. Pogozheva
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
M. Sukonnikov
Faculty of Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 119991
E. Rogozhin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 119021; Moscow, 117997
I. Prokhorenko
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 119021; Moscow, 117997
V. Brylev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 117997
A. Korolev
Gause Institute of New Antibiotics
Email: ap2rin@gmail.com
Rússia, Moscow, 119021
M. Slyundina
Gause Institute of New Antibiotics; Topchiev Institute of Petrochemical Synthesis
Email: ap2rin@gmail.com
Rússia, Moscow, 119021; Moscow, 119991
R. Borisov
Topchiev Institute of Petrochemical Synthesis; Faculty of Physics, Mathematics and Natural Sciences
Email: ap2rin@gmail.com
Rússia, Moscow, 119991; Moscow, 115419
M. Serebryakova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119992
M. Shuvalov
Gause Institute of New Antibiotics; Faculty of Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 119021; Moscow, 119991
A. Ksenofontov
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119992
L. Stoyanova
Faculty of Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119991
I. Osterman
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119991; Moscow, 119992
A. Formanovsky
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 117997
V. Tashlitsky
Faculty of Chemistry
Email: ap2rin@gmail.com
Rússia, Moscow, 119991
L. Baratova
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119992
A. Timofeeva
Belozersky Institute of Physico-Chemical Biology
Email: ap2rin@gmail.com
Rússia, Moscow, 119992
A. Tyurin
Gause Institute of New Antibiotics; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Autor responsável pela correspondência
Email: ap2rin@gmail.com
Rússia, Moscow, 119021; Moscow, 117997
