Chromatographic and Mass-Spectrometric Identification of Linear and Cyclic Peptides Based on Glycine
- Авторы: Milyushkin A.1, Matyushin D.1, Buryak A.1
-
Учреждения:
- Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
- Выпуск: Том 73, № 10 (2018)
- Страницы: 999-1002
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182851
- DOI: https://doi.org/10.1134/S1061934818100064
- ID: 182851
Цитировать
Аннотация
The retention of glycine-based peptides on a Hypercarb carbon adsorbent is studied by reversed-phase HPLC. In the reaction mixture obtained in the synthesis of polyglycines, ions corresponding to both linear and cyclic peptides are detected by electrospray ionization mass spectrometry. Characteristics of the retention of the components are determined on varying the composition of the mobile phase in the isocratic mode. It is shown that some of the detected ions are fragmented ions of linear polyglycines rather than protonated individual cyclic peptides, which complicated identification. The presence of five linear polyglycines as well as cyclodiglycine in the test mixture is confirmed.
Ключевые слова
Об авторах
A. Milyushkin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: lex-miles@yandex.ru
Россия, Moscow, 119071
D. Matyushin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Россия, Moscow, 119071
A. Buryak
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Россия, Moscow, 119071
![](/img/style/loading.gif)