Chromatographic and Mass-Spectrometric Identification of Linear and Cyclic Peptides Based on Glycine
- 作者: Milyushkin A.1, Matyushin D.1, Buryak A.1
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隶属关系:
- Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
- 期: 卷 73, 编号 10 (2018)
- 页面: 999-1002
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182851
- DOI: https://doi.org/10.1134/S1061934818100064
- ID: 182851
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详细
The retention of glycine-based peptides on a Hypercarb carbon adsorbent is studied by reversed-phase HPLC. In the reaction mixture obtained in the synthesis of polyglycines, ions corresponding to both linear and cyclic peptides are detected by electrospray ionization mass spectrometry. Characteristics of the retention of the components are determined on varying the composition of the mobile phase in the isocratic mode. It is shown that some of the detected ions are fragmented ions of linear polyglycines rather than protonated individual cyclic peptides, which complicated identification. The presence of five linear polyglycines as well as cyclodiglycine in the test mixture is confirmed.
作者简介
A. Milyushkin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: lex-miles@yandex.ru
俄罗斯联邦, Moscow, 119071
D. Matyushin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
俄罗斯联邦, Moscow, 119071
A. Buryak
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
俄罗斯联邦, Moscow, 119071
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