Chromatographic and Mass-Spectrometric Identification of Linear and Cyclic Peptides Based on Glycine
- Authors: Milyushkin A.L.1, Matyushin D.D.1, Buryak A.K.1
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Affiliations:
- Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
- Issue: Vol 73, No 10 (2018)
- Pages: 999-1002
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182851
- DOI: https://doi.org/10.1134/S1061934818100064
- ID: 182851
Cite item
Abstract
The retention of glycine-based peptides on a Hypercarb carbon adsorbent is studied by reversed-phase HPLC. In the reaction mixture obtained in the synthesis of polyglycines, ions corresponding to both linear and cyclic peptides are detected by electrospray ionization mass spectrometry. Characteristics of the retention of the components are determined on varying the composition of the mobile phase in the isocratic mode. It is shown that some of the detected ions are fragmented ions of linear polyglycines rather than protonated individual cyclic peptides, which complicated identification. The presence of five linear polyglycines as well as cyclodiglycine in the test mixture is confirmed.
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About the authors
A. L. Milyushkin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Author for correspondence.
Email: lex-miles@yandex.ru
Russian Federation, Moscow, 119071
D. D. Matyushin
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Russian Federation, Moscow, 119071
A. K. Buryak
Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Russian Federation, Moscow, 119071