A “Low-Molecular” Approach to the Identification of Microorganisms by MALDI Mass Spectrometry
- Авторлар: Milman B.1, Ilyasov Y.1, Lugovkina N.1,2, Golovina A.1, Dmitriev A.1
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Мекемелер:
- Institute of Experimental Medicine
- Institute of Toxicology
- Шығарылым: Том 72, № 13 (2017)
- Беттер: 1280-1288
- Бөлім: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182707
- DOI: https://doi.org/10.1134/S1061934817130068
- ID: 182707
Дәйексөз келтіру
Аннотация
A new approach to the identification of microorganisms is presented. It includes the transformation of their MALDI mass spectra aimed at reducing mass scale by one order of magnitude and the use of standard software for building mass spectral libraries of low-molecular compounds and library searches. A library of 728 transformed (“rescaled”) mass spectra of 182 strains for some Streptococcus species was built. With this library, the rate of true microorganism identification was estimated by cross-comparison between library mass spectra (internal validation of the approach). The true identification rate was 84% for three Streptococcus species, which corresponds to the average trueness of species identification by MALDI as found in the literature. The proposed approach to identification can be considered as a method of choice for solving identification problems under consideration.
Негізгі сөздер
Авторлар туралы
B. Milman
Institute of Experimental Medicine
Хат алмасуға жауапты Автор.
Email: bmilman@mail.rcom.ru
Ресей, St. Petersburg, 197376
Yu. Ilyasov
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Ресей, St. Petersburg, 197376
N. Lugovkina
Institute of Experimental Medicine; Institute of Toxicology
Email: bmilman@mail.rcom.ru
Ресей, St. Petersburg, 197376; St. Petersburg, 192019
A. Golovina
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Ресей, St. Petersburg, 197376
A. Dmitriev
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Ресей, St. Petersburg, 197376