A “Low-Molecular” Approach to the Identification of Microorganisms by MALDI Mass Spectrometry
- Authors: Milman B.L.1, Ilyasov Y.Y.1, Lugovkina N.V.1,2, Golovina A.A.1, Dmitriev A.V.1
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Affiliations:
- Institute of Experimental Medicine
- Institute of Toxicology
- Issue: Vol 72, No 13 (2017)
- Pages: 1280-1288
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182707
- DOI: https://doi.org/10.1134/S1061934817130068
- ID: 182707
Cite item
Abstract
A new approach to the identification of microorganisms is presented. It includes the transformation of their MALDI mass spectra aimed at reducing mass scale by one order of magnitude and the use of standard software for building mass spectral libraries of low-molecular compounds and library searches. A library of 728 transformed (“rescaled”) mass spectra of 182 strains for some Streptococcus species was built. With this library, the rate of true microorganism identification was estimated by cross-comparison between library mass spectra (internal validation of the approach). The true identification rate was 84% for three Streptococcus species, which corresponds to the average trueness of species identification by MALDI as found in the literature. The proposed approach to identification can be considered as a method of choice for solving identification problems under consideration.
Keywords
About the authors
B. L. Milman
Institute of Experimental Medicine
Author for correspondence.
Email: bmilman@mail.rcom.ru
Russian Federation, St. Petersburg, 197376
Yu. Yu. Ilyasov
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Russian Federation, St. Petersburg, 197376
N. V. Lugovkina
Institute of Experimental Medicine; Institute of Toxicology
Email: bmilman@mail.rcom.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 192019
A. A. Golovina
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Russian Federation, St. Petersburg, 197376
A. V. Dmitriev
Institute of Experimental Medicine
Email: bmilman@mail.rcom.ru
Russian Federation, St. Petersburg, 197376