HPLC—high-resolution time-of-flight mass spectrometry: Identification and determinarion of β-agonists in food and feed
- Authors: Amelin V.G.1, Korotkov A.I.1,2, Andoralov A.M.1,2
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Affiliations:
- Vladimir State University
- Bryansk Interregional Veterinary Laboratory
- Issue: Vol 71, No 6 (2016)
- Pages: 598-604
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181788
- DOI: https://doi.org/10.1134/S1061934816060034
- ID: 181788
Cite item
Abstract
A method is proposed for the simultaneous identification and determination of 19 β-agonists by exact ion masses in feed, meat, liver, and kidneys of animals using high-resolution time-of-flight mass spectrometry combined with HPLC and simple and fast sample preparation. Matrix effects under the conditions of electrospray ionization were eliminated by the dilution of the extract with a 10-fold volume of deionized water. A scheme of the identification and determination of analytes by the standard addition method is proposed. The limits of detection are 0.11–10 ng/g and limits of quantification, 0.34–31 ng/g. The relative standard deviation of the results of analysis does not exceed 13%. The time of analysis is 1–2 h.
About the authors
V. G. Amelin
Vladimir State University
Author for correspondence.
Email: amelinvg@mail.ru
Russian Federation, ul. Gor’kogo 87, Vladimir, 600000
A. I. Korotkov
Vladimir State University; Bryansk Interregional Veterinary Laboratory
Email: amelinvg@mail.ru
Russian Federation, ul. Gor’kogo 87, Vladimir, 600000; ul. Shosseinaya 7, Bryansk, 241520
A. M. Andoralov
Vladimir State University; Bryansk Interregional Veterinary Laboratory
Email: amelinvg@mail.ru
Russian Federation, ul. Gor’kogo 87, Vladimir, 600000; ul. Shosseinaya 7, Bryansk, 241520