HPLC—high-resolution time-of-flight mass spectrometry: Identification and determinarion of β-agonists in food and feed

Abstract

A method is proposed for the simultaneous identification and determination of 19 β-agonists by exact ion masses in feed, meat, liver, and kidneys of animals using high-resolution time-of-flight mass spectrometry combined with HPLC and simple and fast sample preparation. Matrix effects under the conditions of electrospray ionization were eliminated by the dilution of the extract with a 10-fold volume of deionized water. A scheme of the identification and determination of analytes by the standard addition method is proposed. The limits of detection are 0.11–10 ng/g and limits of quantification, 0.34–31 ng/g. The relative standard deviation of the results of analysis does not exceed 13%. The time of analysis is 1–2 h.