An Improved Procedure for the Gas Chromatography–Tandem Mass Spectrometry Detection of the Globin Adduct of Sulfur Mustard

Abstract

Features of the electron and chemical ionization mass spectra of derivatives of N-[2-[(hydroxyethyl)thio]ethyl]-DL-valine (НЕТЕ-Val)—a biomarker of sulfur mustard—recorded by GC–MS and GC–MS/MS are studied. The retention indices of the studied derivatives were measured. An optimized procedure of the GC–MS/MS (MRM mode) identification of НЕТЕ-Val was tested in the analysis of donor blood samples in vitro exposed to sulfur mustard. The linear range of the detector was from 10 to 100 ng mL^–1, and the limit of detection for sulfur mustard was 10 ng mL^–1.