Determination of muscular tissue proteins by 2D electrophoresis and time-of-flight mass spectrometry

N. L. Vostrikova; A. V. Kulikovskii; I. M. Chernukha; L. I. Kovalev; S. A. Savchuk

doi:10.1134/S1061934817100173

Determination of muscular tissue proteins by 2D electrophoresis and time-of-flight mass spectrometry

Authors: Vostrikova N.L.¹, Kulikovskii A.V.¹, Chernukha I.M.¹, Kovalev L.I.², Savchuk S.A.³
Affiliations:
1. Gorbatov All-Russia Meat Research Institute
2. Federal Research Center of Biotechnology
3. Institute of Pharmacy and Translational Medicine
Issue: Vol 72, No 10 (2017)
Pages: 1102-1112
Section: Articles
URL: https://journals.rcsi.science/1061-9348/article/view/182662
DOI: https://doi.org/10.1134/S1061934817100173
ID: 182662

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Abstract

A procedure is developed for the determination of meat proteins by two-dimensional electrophoresis (2D). The procedure is intended for the quantitative determination of muscle proteins in samples of meat raw materials by a complex of proteomic technologies, including 2D-electrophoresis in a polyacrylamide gel and computer densitometry of the recorded electropherograms.