Determination of muscular tissue proteins by 2D electrophoresis and time-of-flight mass spectrometry

N. L. Vostrikova; A. V. Kulikovskii; I. M. Chernukha; L. I. Kovalev; S. A. Savchuk

doi:10.1134/S1061934817100173

Determination of muscular tissue proteins by 2D electrophoresis and time-of-flight mass spectrometry

Autores: Vostrikova N.¹, Kulikovskii A.¹, Chernukha I.¹, Kovalev L.², Savchuk S.³
Afiliações:
1. Gorbatov All-Russia Meat Research Institute
2. Federal Research Center of Biotechnology
3. Institute of Pharmacy and Translational Medicine
Edição: Volume 72, Nº 10 (2017)
Páginas: 1102-1112
Seção: Articles
URL: https://journals.rcsi.science/1061-9348/article/view/182662
DOI: https://doi.org/10.1134/S1061934817100173
ID: 182662

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Resumo

A procedure is developed for the determination of meat proteins by two-dimensional electrophoresis (2D). The procedure is intended for the quantitative determination of muscle proteins in samples of meat raw materials by a complex of proteomic technologies, including 2D-electrophoresis in a polyacrylamide gel and computer densitometry of the recorded electropherograms.