Email this article Development of Pyrosequencing Assay for Evaluation of Genetic Stability of Vaccine Strains of Live Attenuated Influenza Type B Vaccine
- Authors: Stepanova E.А.1, Krutikova E.V.1, Kiseleva I.V.1, Vasil’eva V.A.1, Rudenko L.G.1
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Affiliations:
- A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
- Issue: Vol 165, No 2 (2018)
- Pages: 243-247
- Section: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/240198
- DOI: https://doi.org/10.1007/s10517-018-4139-1
- ID: 240198
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Abstract
We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be used for analysis of genetic stability of reassortants during vaccine strain development and manufacturing, as well as genetic stability of vaccine isolates of influenza B virus during pre-clinical and clinical trials.
About the authors
E. А. Stepanova
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Author for correspondence.
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
E. V. Krutikova
Email: fedorova.iem@gmail.com
I. V. Kiseleva
Email: fedorova.iem@gmail.com
V. A. Vasil’eva
Email: fedorova.iem@gmail.com
L. G. Rudenko
Email: fedorova.iem@gmail.com
