对比不同方法获取的用于治疗黄斑裂孔患者自体血浆的参数

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

课题迫切性：近年来针对黄斑裂孔患者的治疗越来越多的采用自体血浆疗法。在这项研究中，对通过不同方法获取的自体血浆进行了生物分析。

研究目的：对比不同方法所获取的用于治疗黄斑裂孔患者自体血浆的细胞和生化成分。

材料和方法：对24名患者血浆中的血小板、白细胞和纤维蛋白原的数量进行了研究，该血浆成分通过原始自体血浆收集系统和实验室试管离心法获取。

结果：通过对比Arthrex ACP制备器和实验室试管获取的P-PRP值，血小板和纤维蛋白的数值在统计学上无显著差异（p<0.05），白细胞的数量在统计学上有显著差异（p>0.05）,实验室试管的基质中白细胞数量增加。通过对比Ycellbio-Kit制备器和实验室试管得到的L-PRP值，纤维蛋白原的数量在统计学上无差异（p<0.05），白细胞和血小板的含量在统计学上有显著差异（p>0.05），实验室试管得到的自体血浆浓度低。

结论：目前通过Arthrex ACP制备器获取的自体血浆由于其极少的白细胞含量、系统密封性以及所得基质具有更好的凝固特性在黄斑裂孔外科治疗中备受关注。实验室试管被认为是获取用于治疗黄斑裂孔的自体血浆（P-PRP）更实惠的替代方案。通过Ycellbio-Kit制备器和实验室试管获取的自体血浆（L-PRP）在成分上不太适用于黄斑裂孔的外科治疗。

作者简介

Evgeniy M. Popov

S.M. Kirov Medical Military Academy

Email: popov138army@mail.ru
ORCID iD: 0000-0002-6606-7027
SPIN 代码: 6909-0299

Adjunct of the Department of ophthalmology

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Alexei N. Kulikov

S.M. Kirov Medical Military Academy

Email: alexey.kulikov@mail.ru
ORCID iD: 0000-0002-5274-6993
SPIN 代码: 6440-7706

Dr. Sci. (Med.), Associate Professor, Head of the Department of ophthalmology

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Sergey V. Churashov

S.M. Kirov Medical Military Academy

Email: churashoff@mail.ru
ORCID iD: 0000-0003-1197-9237
SPIN 代码: 5370-7410

Dr. Sci. (Med.), Professor of the Department of ophthalmology

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Il’ya O. Gavrilyuk

S.M. Kirov Medical Military Academy

Email: gavriluik.vma@gmail.com
ORCID iD: 0000-0003-0932-2818
SPIN 代码: 9649-7311

Cand. Sci. (Med.), Lecturer of the Ophthalmology Department

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Elisaveta N. Egorova

S.M. Kirov Medical Military Academy

Email: egorovaelisaveta@mail.ru
ORCID iD: 0000-0002-5272-361X

Cand. Sci. (Med.), Head of the Biochemical Laboratory

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Anastasiya I. Abbasova

S.M. Kirov Medical Military Academy

编辑信件的主要联系方式.
Email: 3363939@mail.ru
ORCID iD: 0000-0002-6850-4737

Doctor of clinical laboratory diagnostics of the clinical laboratory

俄罗斯联邦, 6, Akademika Lebedeva st., Saint Petersburd, 194044

参考

  1. Degenhardt V, Busch C, Jochmann C, et al. Prognostic factors in patients with persistent full-thickness idiopathic macular holes treated with re-vitrectomy with autologous platelet concentrate. Ophthalmologica. 2019;242(4):214–221. doi: 10.1159/000502386
  2. Shpak AA, Shkvorchenko DO, Krupina EA. Surgical treatment of macular holes with and without the use of autologous platelet-rich plasma. Int Ophthalmol. 2021;41(3):1043–1052. doi: 10.1007/s10792-020-01662-4
  3. Babu N, Kohli P, Ramachandran NO, et al. Comparison of platelet-rich plasma and inverted internal limiting membrane flap for the management of large macular holes: A pilot study. Indian J Ophthalmol. 2020;68(5):880–884. doi: 10.4103/ijo.IJO_1357_19
  4. Fayzrahmanov RR, Krupina EA, Pavlovskiy OA, et al. Analysis of platelet-rich plasma obtained in various ways. Medline. ru. Rossiiskii biomeditsinskii zhurnal 2019;20(2):363–372. (In Russ.)
  5. Shkvorchenko DO, Zakharov VD, Krupina EA, et al. Surgical treatment of primary macular hole using platelet-rich plasma. Fyodorov Journal of Ophthalmic Surgery. 2017;(3):27–30. (In Russ.) doi: 10.25276/0235-4160-2017-3-27-30
  6. Arsyutov DG. The use of autologous conditioned platelet rich plasma in the surgery of rhegmatogenous retinal detachment with central, paracentral and peripheral tears. Saratov journal of Medical Scientific Research. 2019;15(2):422–425. (In Russ.)
  7. Kuznetsova NV, Kurenkov VV, Kurenkova NV, Abramov SI. Ispol’zovanie PRP-tekhnologii v konservativnom lechenii diffuznogo lamellyarnogo keratita posle Lasik. Modern technologies in ophthalmology. 2017;(6):183–185. (In Russ.)
  8. Tarabrina VA, Gavrilyuk IO, Churashov SV, Kulikov AN. The use of platelet-rich plasma on the experimental corneal erosion. Modern technologies in ophthalmology. 2020;(3):83–84. (In Russ.) doi: 10.25276/2312-4911-2020-3-83-84
  9. Kulikov AN, Churashov SV, Popov EM. Methods of treatment of macular hole: history and prospects. Bulletin of Pirogov National Medical & Surgical Center. 2021;16(1):135–138. (In Russ.) doi: 10.25881/BPNMSC.2021.14.53.026
  10. Bikbov MM, Zainullin RM, Gilmanshin TR, et al. The results of large macular hole surgery using Autologous conditioned plasma. Point of view. East–West. 2020;(2):33–35. (In Russ.) doi: 10.25276/2410-1257-2020-2-33-35
  11. Petrachkov DV, Alkharki L, Matyushchenko AG, et al. Comparison of Early Treatment Outcomes for Large Macular Hole Using Various Surgical Techniques. Ophthalmology in Russia. 2021;18(3S):681–687. (In Russ.) doi: 10.18008/1816-5095-2021-3S-681-687
  12. Shkvorchenko DO, Zakharov VD, Krupina EA, et al. Surgical treatment of primary macular hole using platelet-rich plasma. Fyodorov Journal of Ophthalmic Surgery. 2017;(3):27–30. (In Russ.) doi: 10.25276/0235-4160-2017-3-27-30
  13. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: From pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158–167. doi: 10.1016/j.tibtech.2008.11.009
  14. Arthrex.com [Internet]. Instruktsiya po primeneniyu sistemy Arthrex ACP shprits v shpritse firmy Arthrex [cited: 2021 Nov 23]. Available from: https://www.arthrex.com/resources/brochures/pF8_4Jzo7U2V9AF47os7pg/arthrex-acp-double-syringe-hettich-centrifuge (In Russ.)
  15. PRPLab [Internet]. Instruktsiya po primeneniyu sistemy Ycellbio-Kit firmy Vaisellbiomedikal [cited: 2021 Nov 23]. Available from: http://www.prplab.ru/metod-prp.htm (In Russ.)
  16. Dolgov VV, Men’shikov VV. Klinicheskaya laboratornaya diagnostika: natsional’noe rukovodstvo, Vol. 1. Moscow: GEHOTAR-Media, 2012. 928 p. (In Russ.)
  17. Arsyutov DG. The use of a new type of platelet-rich plasma — autologous conditioned plasma (ACP) in the surgery of rhegmatogenous retinal detachment with large and multiple breaks, abruption from the dentate line. Modern technologies in ophthalmology. 2019;(1):22–25. (In Russ.) doi: 10.25276/2312-4911-2019-1-22-25
  18. Zakharov VD, Shkvorchenko DO, Krupina EA, et al. Efficacy of platelet-rich plasma in surgery of large macular raptures. Practical medicine. 2016;(9):118–121. (In Russ.)
  19. Fedoseeva EV, Chentsova EV, Borovkova NV, et al. Morphofunctional Peculiarities of Platelet Rich Plasma and Its Application in Ophthalmology. Ophthalmology in Russia. 2018;15(4):388–393. (In Russ.) doi: 10.18008/1816-5095-2018-4-388-393
  20. Krupina EA, Fajzrahmanov RR, Pavlovskij OA, et al. Molecular and biological аspects of platelet-rich plasma therapies. Bulletin of Pirogov National Medical & Surgical Center. 2020;15(3–2):80–85. (In Russ.) doi: 10.25881/BPNMSC.2020.30.34.015

补充文件

附件文件
动作
1. JATS XML
下载
2. 图1.用于自体调节血浆的Rotofix 32A离心机和Arthrex ACP系统Fig. 1. The Rotofix 32A centrifuge and the Arthrex ACP system for producing autologous conditioned serum

下载 (135KB)
索引源数据
3. 图2.用于制备富含血小板自体血浆的多功能离心机CM 6M和实验室试管Fig. 2. Multi Centrifuge CM 6M centrifuge and laboratory test tube for producing platelet-rich autologous serum

下载 (129KB)
索引源数据
4. 图.3.用于制备富含血小板的自体血浆的Armed 80-2S离心机和Ycellbio-Kit系统 Fig. 3. The Armed 80-2S centrifuge and the Ycellbio-Kit system for producing platelet-rich autologous serum

下载 (111KB)
索引源数据
5. 图4.沉淀的血液细胞成分（用箭头表示）和获得的 L-PRPFig. 4. Precipitated blood cell elements (indicated by arrow), and obtained L-PRP

下载 (106KB)
索引源数据

版权所有 © Popov E., Kulikov A., Churashov S., Gavrilyuk I., Egorova E., Abbasova A., 2021

Creative Commons License
此作品已接受知识共享署名 4.0国际许可协议的许可
 


##common.cookie##