Comparison of indicators of autologous serum obtained by different methods and used for the treatment of macular hole

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Abstract

BACKGROUND: In recent years, autologous serum has been increasingly used in the treatment of macular hole. The study carried out a biological analysis of autologous serum obtained by various methods.

AIM: The aim of the investigation is to compare the cellular and biochemical composition of autologous serum obtained using different methods for use in the macular hole treatment.

MATERIALS AND METHODS: The study of the number of platelets, leukocytes and fibrinogen level in the blood serum of 24 patients obtained by centrifugation in original systems for harvesting autologous serum and laboratory test tubes was performed.

RESULTS: The indices of P-PRP obtained in the Arthrex ACP system and in a laboratory test tube do not statistically differ in quantitative indices of fibrinogen and platelets (p < 0.05), and differ in the content of leukocytes (p > 0.05) in the direction of increasing the number of leukocytes in the substrate obtained in a laboratory test tube. The indicators of L-PRP obtained in the Ycellbio-Kit system and in a laboratory test tube do not statistically differ in the amount of fibrinogen (p < 0.05) and differ in the content of platelets and leukocytes (p > 0.05) in the direction of decreasing the concentration of leukocytes and platelets in the autologous serum obtained in a laboratory test tube.

CONCLUSIONS: Currently, autologous serum obtained in the Arthrex ACP system is of great interest for macular hole surgery due to the minimal content of leukocytes, the closed character of the system, and the best coagulation properties of the substrate obtained. Laboratory test tubes may be considered a more affordable alternative for the production of autologous serum (P-PRP) for the treatment of macular hole. The autologous serum (L-PRP) obtained in the Ycellbio-Kit system and in a laboratory test tube is less suitable for macular hole surgery according to its composition.

About the authors

Evgeniy M. Popov

S.M. Kirov Medical Military Academy

Email: popov138army@mail.ru
ORCID iD: 0000-0002-6606-7027
SPIN-code: 6909-0299

Adjunct of the Department of ophthalmology

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Alexei N. Kulikov

S.M. Kirov Medical Military Academy

Email: alexey.kulikov@mail.ru
ORCID iD: 0000-0002-5274-6993
SPIN-code: 6440-7706

Dr. Sci. (Med.), Associate Professor, Head of the Department of ophthalmology

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Sergey V. Churashov

S.M. Kirov Medical Military Academy

Email: churashoff@mail.ru
ORCID iD: 0000-0003-1197-9237
SPIN-code: 5370-7410

Dr. Sci. (Med.), Professor of the Department of ophthalmology

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Il’ya O. Gavrilyuk

S.M. Kirov Medical Military Academy

Email: gavriluik.vma@gmail.com
ORCID iD: 0000-0003-0932-2818
SPIN-code: 9649-7311

Cand. Sci. (Med.), Lecturer of the Ophthalmology Department

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Elisaveta N. Egorova

S.M. Kirov Medical Military Academy

Email: egorovaelisaveta@mail.ru
ORCID iD: 0000-0002-5272-361X

Cand. Sci. (Med.), Head of the Biochemical Laboratory

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

Anastasiya I. Abbasova

S.M. Kirov Medical Military Academy

Author for correspondence.
Email: 3363939@mail.ru
ORCID iD: 0000-0002-6850-4737

Doctor of clinical laboratory diagnostics of the clinical laboratory

Russian Federation, 6, Akademika Lebedeva st., Saint Petersburd, 194044

References

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Supplementary files

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2. Fig. 1. The Rotofix 32A centrifuge and the Arthrex ACP system for producing autologous conditioned serum

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3. Fig. 2. Multi Centrifuge CM 6M centrifuge and laboratory test tube for producing platelet-rich autologous serum

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4. Fig. 3. The Armed 80-2S centrifuge and the Ycellbio-Kit system for producing platelet-rich autologous serum

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5. Fig. 4. Precipitated blood cell elements (indicated by arrow), and obtained L-PRP

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Copyright (c) 2021 Popov E.M., Kulikov A.N., Churashov S.V., Gavrilyuk I.O., Egorova E.N., Abbasova A.I.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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