Domestic devices for molecular genetic analysis: developments of the IAP RAS and SINTOL LLC
- 作者: Kurochkin V.E.1, Alekseev Y.I.2, Petrov D.G.1, Evstrapov A.A.1
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隶属关系:
- Institute for Analytical Instrumentation of the Russian Academy of Sciences
- “Syntol” LLC
- 期: 卷 40, 编号 3 (2021)
- 页面: 69-74
- 栏目: Reviews
- URL: https://journals.rcsi.science/RMMArep/article/view/76918
- DOI: https://doi.org/10.17816/rmmar76918
- ID: 76918
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详细
The set of domestic devices and reagent kits for molecular genetic analysis has been developed, which provides for all stages of the study, from the automated isolation of nucleic acids, their amplification with real-time detection to classical and genome-wide DNA sequencing. With the help of the created equipment and reagent kits, approaches for the specific indication of pathogens of a wide range of diseases have been successfully implemented. The high efficiency of the proposed approaches is shown by the example of detecting and analyzing the RNA of the pathogen SARS-CoV-2 in the fight against the COVID-19 coronavirus pandemic (7 figs, bibliography: 4 refs).
作者简介
Vladimir Kurochkin
Institute for Analytical Instrumentation of the Russian Academy of Sciences
Email: lavrovas@yandex.ru
SPIN 代码: 1868-9326
Researcher ID: S-7871-2016
D.Sc. (Тechnical), Professor
俄罗斯联邦, 26, Rizhskiy ave., Saint Petersburg, 190103Yakov Alekseev
“Syntol” LLC
Email: jalex01@mail.ru
ORCID iD: 0000-0002-1696-7684
SPIN 代码: 8145-5586
Ph.D. (Biology)
俄罗斯联邦, MoscowDmitriy Petrov
Institute for Analytical Instrumentation of the Russian Academy of Sciences
Email: dimoon88@mail.ru
SPIN 代码: 2975-8180
Scopus 作者 ID: 761722
俄罗斯联邦, 26, Rizhskiy ave., Saint Petersburg, 190103
Anatoliy Evstrapov
Institute for Analytical Instrumentation of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: an_evs@mail.ru
ORCID iD: 0000-0003-4495-8096
SPIN 代码: 2053-5020
Scopus 作者 ID: 22691
Researcher ID: I-9739-2014
D.Sc. (Тechnical)
俄罗斯联邦, 26, Rizhskiy ave., Saint Petersburg, 190103参考
- Alekseev YaI, Belov YuV, Varlamov DA, et al. Devices for diagnostics of biological objects based on the method of polymerase chain reaction in real time (PCR-RT). Scientific Instrument Engineering. 2006;16(3):132–136. (In Russ.)
- Alekseev YaI, Belov YuV, Malyuchenko OP, et al. Genetic analyzer for DNA fragment analysis. Scientific Instrument Making. 2012;22(4):86–92. (In Russ.)
- Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. Available from: https://www.ncbi.nlm.nih.gov/nuccore/NC_045512 (accessed 13.05.2021).
- Garafutdinov RR, Mavzyutov AR, Alekseev YaI, et al. Betacoronaviruses of humans and their highly sensitive detection using PCR and other methods of amplification. Biomics. 2020;12 (1):121–179. (In Russ.)
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