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Post a Comment On placental erythropoietin expression in abnormal placentation
- Authors: Syundyukova E.G.1,2, Sashenkov S.L.1, Chulanova Y.S.2, Golovneva E.S.1, Chulkov V.S.3, Filippova N.A.1,2, Yakovleva Y.A.1, Tarasova L.B.1
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Affiliations:
- South Ural State Medical University
- Clinic of the South Ural State Medical University
- Yaroslav-the-Wise Novgorod State University
- Issue: Vol 42, No 6 (2025)
- Pages: 70-78
- Section: Original studies
- URL: https://journals.rcsi.science/PMJ/article/view/381419
- DOI: https://doi.org/10.17816/pmj42670-78
- ID: 381419
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Abstract
Objective. To study placental erythropoietin (EPO) expression in placenta previa and placenta accreta (PAS - placenta accreta spectrum).
Materials and methods. A morphological study of placenta was performed in women who delivered at the obstetric department of the Clinic of the South Ural State Medical University (Chelyabinsk, Russia). The sample included 53 placental specimens: group 1 – 21 specimens of normally situated placenta, group 2 – 17 specimens of placenta previa without signs of invasion, group 3 – 15 PAS specimens, comprising 7 cases of placenta accreta, 5 of placenta increta, 3 of placenta percreta (invasion degree 3a). Placental expression was studied using monoclonal antibodies to EPO (EPO antibody Rabbit Polyclonal, suitable for Western blot – WB, immunohistochemistry – IHC-P, and ELISA; Human, U.S.A.), the intensity of the immunohistochemical reaction was assessed using a semi-quantitative 3-point scale.
Results. Placental EPO expression in abnormal placentation was statistically significantly increased in the capillary endothelial cells of villi (group 1 – 1 (1–2); group 2 – 2 (2–2,5); group 3 – 2 (2–3); p < 0,001) showing an increasing trend in PAS. Specifically, in placental invasion, a marked EPO expression was recorded in syncytiotrophoblast (group 1 – 2 (2–2); group 2 – 2 (2–3); group 3 – 3 (2–3); p1-3 < 0,001; p < 0,001) and villous stroma macrophages (group 1 – 2 (1–2); group 2 – 2 (2–2,5); group 3 – 2 (2–3); p = 0,002), while reduced (moderate) expression was observed in amniotic epithelium (group 1 – 3 (2–3); group 2 – 2 (2–3); group 3 – 2 (2–3); p1-3 = 0,016; p = 0,034).
Conclusions. In abnormal placentation, a statistically significant increase was revealed in the number of capillary endothelial cells of placental villi expressing erythropoietin. In PAS, marked erythropoietin expression was observed in syncytiotrophoblast and villous stromal macrophages, with a concurrent decrease in amniotic epithelium. The inclusion of maternal serum erythropoietin concentration measurement in prenatal screening protocols appears promising for assessing the likelihood of placenta previa and placenta accreta.
About the authors
E. G. Syundyukova
South Ural State Medical University; Clinic of the South Ural State Medical University
Email: seg269@mail.ru
ORCID iD: 0000-0001-9535-1871
DSc (Medicine), Associate Professor, Professor of the Department of Obstetrics and Gynecology
Russian Federation, Chelyabinsk; ChelyabinskS. L. Sashenkov
South Ural State Medical University
Email: y.chul@mail.ru
ORCID iD: 0000-0002-6007-1041
DSc (Medicine), Professor, Head of the Department of Normal Physiology named after Yu.M. Zakharov
Russian Federation, ChelyabinskY. S. Chulanova
Clinic of the South Ural State Medical University
Author for correspondence.
Email: y.chul@mail.ru
ORCID iD: 0000-0001-5507-9471
Obstetrician-gynecologist
Russian Federation, ChelyabinskE. S. Golovneva
South Ural State Medical University
Email: y.chul@mail.ru
ORCID iD: 0000-0002-5752-4863
DSc (Medicine), Professor, Professor of the Department of Normal Physiology named after Yu.M. Zakharov
Russian Federation, ChelyabinskV. S. Chulkov
Yaroslav-the-Wise Novgorod State University
Email: vschulkov@rambler.ru
ORCID iD: 0000-0002-0952-6856
DSc (Medicine), Associate Professor, Professor of the Department of Internal Medicine
Russian Federation, Veliky NovgorodN. A. Filippova
South Ural State Medical University; Clinic of the South Ural State Medical University
Email: y.chul@mail.ru
ORCID iD: 0009-0002-8209-2035
PhD (Medicine), Associate Professor of the Department of Obstetrics and Gynecology
Russian Federation, Chelyabinsk; ChelyabinskY. A. Yakovleva
South Ural State Medical University
Email: y.chul@mail.ru
ORCID iD: 0000-0002-7173-6539
PhD (Medicine), Associate Professor, Associate Professor of the Department of Obstetrics and Gynecology
Russian Federation, ChelyabinskL. B. Tarasova
South Ural State Medical University
Email: y.chul@mail.ru
ORCID iD: 0000-0003-3390-0454
PhD (Medicine), Associate Professor, Associate Professor of the Department of Obstetrics and Gynecology
Russian Federation, ChelyabinskReferences
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