ERYTHROPOIESIS IN ERYTHROBLASTIC BONE MARROW ISLETS DURING TOXIGENIC PERIOD AFTER EXPOSURE TO DIFFERENT POLYCHLORINATED BIPHENYLS DOSES

Aim. The aim of the study was to investigate the status of erythron in toxigenic period after the subacute exposure to polychlorinated biphenyls (PChB) with calculation of the absolute quantity and distribution of erythroblastic islets of the rat femur bone marrow by maturity classes. Materials and methods. The model of subacute PChB intoxication was created by introducing the commercial mixture “Sovol” to rats in the volume of 1 ml daily during 28 days using intragastric pump. According to the introduced dose of the preparation, animals were divided into 2 groups: group I (1/2 LD50) - the total dose of PChb for all the period of its introduction was 3000 mg/kg; group II (1/40 LD50) - the total dose of PChB for all the period of its introduction was 150 mg/kg. Vegetable oil in the dose of 1 ml was introduced to the animals of the control group during 28 days, as well. The experimental material was sampled at the days 7, 28, 56 after the completion of the period of subacute PChB intoxication (28 days) for each group of animals including the control. To assess the status of hemostasis in the central component of erythron, the absolute number was calculate and erythroblastic islets (EI) of the femur bone marrow were distributed by maturity classes using Yu.M. Zakharov, I.Yu. Melnikov, A.G. Rassokhin method (1984-1990); functional indices of EI erythropoiesis were estimated with L.V. Vorgovaya and Yu.M. Zakharov method (1990). Results. It was established, as a result of the conducted studies, that in the groups of rats, introduced PChb in the dose of 1/2 and 1/40 LD50, stress reaction in the system of erythropoiesis was observed. Just after the completion of PChB introduction, during two months there occurred an intensive reconstruction of red blood with activation of compensating erythropoiesis Conclusions. The results confirm the damaging effect of different PChB doses that requires constant control of blood in subjects permanently contacting with dioxin-like compounds at production.

Polychlorinated biphenyls, bone marrow, maturity classes of erythroblastic islets, erythropoiesis, amplification of EI erythroblasts, erythropoiesis reconstruction