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Post a Comment Changes in growth characteristics and biofilm-forming activity of Escherichia coli with cholesterol available
- Authors: Bykova L.P.1, Trapeznikov Y.P.1, Godovalov A.P.1
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Affiliations:
- E.A. Vagner Perm State Medical University
- Issue: Vol 36, No 2 (2019)
- Pages: 98-101
- Section: Biology and experimental medicine
- URL: https://journals.rcsi.science/PMJ/article/view/14330
- DOI: https://doi.org/10.17816/pmj36298-101
- ID: 14330
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Abstract
Aim.To assess the growth properties and formation of E. сoli biofilms with different concentrations of cholesterol available.
Materials and methods. E. coli strains were cultivated in the meat-peptone broth with cholesterol added in the concentration of 3, 5, 7 and 9 mmol/l as well as in the nutrient medium without cholesterol. The growth parameters and cholesterol concentration in the samples prior to and after cultivating microorganisms and the effect of cholesterol on biofilm-forming activity of E. coli was determined.
Results. Cholesterol was shown to have no bactericidal effect on E. coli, however, it is able to change the duration of growth phases of the strains that is, possibly, caused by adaptation to the presence of cholesterol and switching of metabolic pathways. Cholesterol has a stimulating effect on accumulation of biomass by the test-strain and its biofilm-forming activity.
Conclusions. In the study performed, the ability of E. coli to metabolize human cholesterol was shown.
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##article.viewOnOriginalSite##About the authors
L. P. Bykova
E.A. Vagner Perm State Medical University
Author for correspondence.
Email: AGodovalov@gmail.com
доцент кафедры микробиологии и вирусологии
Russian Federation, 26, Petropavlovskay street, Perm, 614000Ya. P. Trapeznikov
E.A. Vagner Perm State Medical University
Email: AGodovalov@gmail.com
студент лечебного факультета
Russian Federation, 26, Petropavlovskay street, Perm, 614000A. P. Godovalov
E.A. Vagner Perm State Medical University
Email: AGodovalov@gmail.com
в.н.с
Russian Federation, 26, Petropavlovskay street, Perm, 614000References
- Годовалов А.П., Быкова Л.П., Бадыков И.И. Холестеринметаболизирующая активность клинических штаммов Candida albicans. Боткинские чтения: материалы Всерос. науч.-практ. конф. СПб. 2017; 52–53.
- Трапезников Я.П., Быкова Л.П., Годовалов А.П. Холестеринметаболизирующая активность Staphylococcus aureus. Материалы I Российского микробиологического конгресса. Пущино 2017; 129.
- Хамагаева И.С., Цыбикова А.Х., Замбалова Н.А. Исследование холестеринметаболизирующих свойств пробиотических микроорганизмов. Молочная промышленность 2011; 10: 56.
- Loh L.N., McCarthy E.M.C., Narang P., Khan N.A., Ward T.H. Escherichia coli K1 utilizes host macropinocytic pathways for invasion of brain microvascular endothelial cells. Traffic 2017; 18(11): 733–746.
- O'Toole G.A. Microtiter dish biofilm formation assay. J Vis Exp 2011; 47: 2437.
- Wilkison W.O., Bellr R.M. sn-Glycerol-3-phosphate acyltransferase from Escherichia coli. Biochimica et Biophysica Acta 1997; 1348: 3–9.
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