Email this article FUNCTIONAL DIVERSIFICATION OF THE CAROTENOID-CIS-TRANS-ISOMERASES CrtISO, CrtISO-L1, AND CrtISO-L2 IN TOMATO SPECIES (SOLANUM, SECTION LYCOPERSICON)
- Authors: Efremov G.I.1, Shchennikova A.V.1, Kochieva E.Z.1
-
Affiliations:
- Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences"
- Issue: Vol 508, No 1 (2023)
- Pages: 9-13
- Section: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/135661
- DOI: https://doi.org/10.31857/S2686738922600686
- EDN: https://elibrary.ru/MOKOFP
- ID: 135661
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Abstract
The expression of the genes of carotenoid-cis-trans isomerases CrtISO, CrtISO-L1 and CrtISO-L2 was studied in comparison with the content of carotenoids in tomato species with different ripe fruit colors: green (Solanum habrochaites), yellow (S. cheesmaniae) and red (S. pimpinellifolium and S. lycopersicum). More ancient origin of CrtISO-L2 was shown in relation to CrtISO and CrtISO-L1. A similar content of total carotenoids (leaves) and β-carotene (ripe fruits) was found between the samples. Unlike fruits of S. habrochaites and S. cheesmaniae, red fruits accumulated lycopene and 20-30 times more total carotenoids. The highest level of transcripts both in leaves and in ripe fruits was detected for CrtISO. The CrtISO-L1 and CrtISO-L2 were transcribed high in leaves and low in fruits, except for the high expression of CrtISO-L2 in S. lycopersicum fruits. No relationship was observed between the content of carotenoids and the level of gene expression in the fruit. In the leaves, a positive correlation between the amount of carotenoids and the levels of CrtISO-L1 and CrtISO-L2 transcripts was found.
About the authors
G. I. Efremov
Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences"
Author for correspondence.
Email: gleb_efremov@mail.ru
Russian Federation, Moscow
A. V. Shchennikova
Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences"
Email: gleb_efremov@mail.ru
Russian Federation, Moscow
E. Z. Kochieva
Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences"
Email: gleb_efremov@mail.ru
Russian Federation, Moscow
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