Molecular marking in a white cabbage selection for resistance to Alternaria Blight

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In the presented study, the polymorphism of SSR loci was studied on the basis of the resistance of white cabbage to alternariosis. This work was carried out in order to identify effective and informative ISSR markers for the identification of donor alleles of resistance to alternariosis in the genotypes of hybrid plants of culture. Of the 20 tested markers, only 3 (PBCESSRJU13, PBCESSRJU6, NI-F02a) demonstrate an allelic difference between the isogenic lines of white cabbage contrasting in resistance to Alternaria Blight. They will become the basis for subsequent studies on the analysis of cleavage in segregating populations and the determination of informative DNA marker systems co-inherited with the sign of resistance of cabbage to alternariosis.

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作者简介

E. Dubina

Federal Scientific Rice Centre; Kuban State Agrarian University named after I.T. Trubilin

编辑信件的主要联系方式.
Email: lenakrug1@rambler.ru

Grand PhD in Biological Sciences, Professor of the RAS

俄罗斯联邦, Krasnodar; Krasnodar

Yu. Makukha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

PhD in Biological Sciences

俄罗斯联邦, Krasnodar

S. Garkusha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Corresponding Member of the RAS

俄罗斯联邦, Krasnodar

D. Simonenko

Kuban State Agrarian University named after I.T. Trubilin

Email: lenakrug1@rambler.ru

PhD Student

俄罗斯联邦, Krasnodar

O. Gorun

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

俄罗斯联邦, Krasnodar

S. Lesnyak

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

俄罗斯联邦, Krasnodar

参考

  1. Kutlunina N.A., Ermoshin A.A. Molekulyarno-geneticheskie metody v issledovanii rasteniy. Ekaterinburg, 2017. 142 s.
  2. Makukha Yu.A., Dubina E.V. Razrabotka metodologii otsenki ustoichivosti kapusty belokochanoy k Xanthomonas campestris s primeneniem SSR-markerov. Risovodstvo. 2019. № 3 (44). С. 27–32.
  3. Doullah M.A.U., Meah M.B., Okazaki K. Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leaf spot) in Brassica rapa. Eur. J. Plant. Pathol. 2006; 116 (1): 33–43. https://doi.org/10.1007/s10658-006-9035-2
  4. Ghosh S., Mazumder M., Mondal B. et al. Morphological and SSR marker-based genetic diversity analysis of Indian mustard (Brassica juncea L.) differing in Alternaria brassicicola tolerance. Euphytica. 2019. V. 215. P. 206–224. https://doi.org/10.1007/s10681-019-2523-1
  5. Hopkins C.J., Cogan N.O.I., Hand M. et al. Sixteen new simple sequence repeat markers from Brassica juncea expressed sequences and their cross-species amplification. Mol. Ecol. Notes. 2007. V. 7 (4). P. 697–700. https://doi.org/10.1111/j.1471-8286.2007.01681.x
  6. Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980. V. 10. P. 4321–4325.
  7. Nowicki M., Nowakowska M., Niezgoda A., Kozik E. Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Veg. Crops Res. Bull. 2012. V. 76 (1). P. 5–19. https://doi.org/10.2478/v10032-012-0001-6
  8. Pratap P., Thakur A.K., Meena P.D. et al. Genetic diversity assessment in Indian mustard (Brassica juncea L.) for Alternaria black leaf spot tolerance using SSR markers. J. Oilseed Brassica. 2015. V. 6 (1). P. 175–182.

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2. Fig. 1. Visualization of PCR products at the PBCESSRJU13 locus in 8% PAGE. Mt, molecular weight marker; S, isogenic stable line 42; U/S, isogenic unstable line 32 (same in Figs. 2,3)

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3. Fig. 2. Visualization of PCR products at the PBCESSRJU6 locus in 8% PAGE

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4. Fig. 3. Visualization of PCR products at the NI-F02a locus in 8% PAGE

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