Enviar artigo por via de e-mail Molecular marking in a white cabbage selection for resistance to Alternaria Blight
- Autores: Dubina E.1,2, Makukha Y.1, Garkusha S.1, Simonenko D.2, Gorun O.1, Lesnyak S.1
-
Afiliações:
- Federal Scientific Rice Centre
- Kuban State Agrarian University named after I.T. Trubilin
- Edição: Nº 2 (2024)
- Páginas: 8-10
- Seção: Crop Production and Selection
- URL: https://journals.rcsi.science/2500-2082/article/view/255689
- DOI: https://doi.org/10.31857/S2500208224020029
- ID: 255689
Citar
Acesso aberto
Acesso está concedido
Resumo
In the presented study, the polymorphism of SSR loci was studied on the basis of the resistance of white cabbage to alternariosis. This work was carried out in order to identify effective and informative ISSR markers for the identification of donor alleles of resistance to alternariosis in the genotypes of hybrid plants of culture. Of the 20 tested markers, only 3 (PBCESSRJU13, PBCESSRJU6, NI-F02a) demonstrate an allelic difference between the isogenic lines of white cabbage contrasting in resistance to Alternaria Blight. They will become the basis for subsequent studies on the analysis of cleavage in segregating populations and the determination of informative DNA marker systems co-inherited with the sign of resistance of cabbage to alternariosis.
Palavras-chave
Texto integral
Sobre autores
E. Dubina
Federal Scientific Rice Centre; Kuban State Agrarian University named after I.T. Trubilin
Autor responsável pela correspondência
Email: lenakrug1@rambler.ru
Grand PhD in Biological Sciences, Professor of the RAS
Rússia, Krasnodar; KrasnodarYu. Makukha
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
PhD in Biological Sciences
Rússia, KrasnodarS. Garkusha
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Corresponding Member of the RAS
Rússia, KrasnodarD. Simonenko
Kuban State Agrarian University named after I.T. Trubilin
Email: lenakrug1@rambler.ru
PhD Student
Rússia, KrasnodarO. Gorun
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Junior Researcher
Rússia, KrasnodarS. Lesnyak
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Junior Researcher
Rússia, KrasnodarBibliografia
- Kutlunina N.A., Ermoshin A.A. Molekulyarno-geneticheskie metody v issledovanii rasteniy. Ekaterinburg, 2017. 142 s.
- Makukha Yu.A., Dubina E.V. Razrabotka metodologii otsenki ustoichivosti kapusty belokochanoy k Xanthomonas campestris s primeneniem SSR-markerov. Risovodstvo. 2019. № 3 (44). С. 27–32.
- Doullah M.A.U., Meah M.B., Okazaki K. Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leaf spot) in Brassica rapa. Eur. J. Plant. Pathol. 2006; 116 (1): 33–43. https://doi.org/10.1007/s10658-006-9035-2
- Ghosh S., Mazumder M., Mondal B. et al. Morphological and SSR marker-based genetic diversity analysis of Indian mustard (Brassica juncea L.) differing in Alternaria brassicicola tolerance. Euphytica. 2019. V. 215. P. 206–224. https://doi.org/10.1007/s10681-019-2523-1
- Hopkins C.J., Cogan N.O.I., Hand M. et al. Sixteen new simple sequence repeat markers from Brassica juncea expressed sequences and their cross-species amplification. Mol. Ecol. Notes. 2007. V. 7 (4). P. 697–700. https://doi.org/10.1111/j.1471-8286.2007.01681.x
- Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980. V. 10. P. 4321–4325.
- Nowicki M., Nowakowska M., Niezgoda A., Kozik E. Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Veg. Crops Res. Bull. 2012. V. 76 (1). P. 5–19. https://doi.org/10.2478/v10032-012-0001-6
- Pratap P., Thakur A.K., Meena P.D. et al. Genetic diversity assessment in Indian mustard (Brassica juncea L.) for Alternaria black leaf spot tolerance using SSR markers. J. Oilseed Brassica. 2015. V. 6 (1). P. 175–182.
Arquivos suplementares
