Molecular marking in a white cabbage selection for resistance to Alternaria Blight

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Abstract

In the presented study, the polymorphism of SSR loci was studied on the basis of the resistance of white cabbage to alternariosis. This work was carried out in order to identify effective and informative ISSR markers for the identification of donor alleles of resistance to alternariosis in the genotypes of hybrid plants of culture. Of the 20 tested markers, only 3 (PBCESSRJU13, PBCESSRJU6, NI-F02a) demonstrate an allelic difference between the isogenic lines of white cabbage contrasting in resistance to Alternaria Blight. They will become the basis for subsequent studies on the analysis of cleavage in segregating populations and the determination of informative DNA marker systems co-inherited with the sign of resistance of cabbage to alternariosis.

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About the authors

E. V. Dubina

Federal Scientific Rice Centre; Kuban State Agrarian University named after I.T. Trubilin

Author for correspondence.
Email: lenakrug1@rambler.ru

Grand PhD in Biological Sciences, Professor of the RAS

Russian Federation, Krasnodar; Krasnodar

Yu. A. Makukha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

PhD in Biological Sciences

Russian Federation, Krasnodar

S. V. Garkusha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Corresponding Member of the RAS

Russian Federation, Krasnodar

D. S. Simonenko

Kuban State Agrarian University named after I.T. Trubilin

Email: lenakrug1@rambler.ru

PhD Student

Russian Federation, Krasnodar

O. L. Gorun

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

Russian Federation, Krasnodar

S. A. Lesnyak

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

Russian Federation, Krasnodar

References

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Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. Visualization of PCR products at the PBCESSRJU13 locus in 8% PAGE. Mt, molecular weight marker; S, isogenic stable line 42; U/S, isogenic unstable line 32 (same in Figs. 2,3)

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3. Fig. 2. Visualization of PCR products at the PBCESSRJU6 locus in 8% PAGE

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4. Fig. 3. Visualization of PCR products at the NI-F02a locus in 8% PAGE

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