Email this article Search for informative marker systems associated with loci of resistance to vascular bacteriosis in cultivated cabbage
- Authors: Dubina E.V.1, Makukha Y.A.1, Garkusha S.V.1, Gorun O.L.1, Lesnyak S.A.1
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Affiliations:
- Federal Scientific Rice Centre
- Issue: No 1 (2024)
- Pages: 15-18
- Section: Crop Production and Selection
- URL: https://journals.rcsi.science/2500-2082/article/view/255760
- DOI: https://doi.org/10.31857/S2500208224010034
- ID: 255760
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Abstract
This article presents the results of studies on the determination of informative DNA marker systems that provide reliable control of the presence of Xcc-loci of resistance to black rot in the breeding material of white cabbage. At the initial stage of the work, 20 molecular markers taken from the VegMarks database were tested on isogenic cabbage lines contrasting in resistance to black rot (resistant line 269-Yas12p-2 and susceptible line Pi714). It was found that only the SSR marker Ol10-C01 reveals polymorphism between contrasting samples of white cabbage. PCR analysis with the use of this polymorphic marker and phytopathological testing have been also performed on F2 plants of the hybrid combination 269-Yas12p-2 × Pi714. As a result of the statistical analysis of cleavage, it was found that the SSR marker Ol10-C01 is co-inherited with a trait of resistance to black rot, since the expected segregation of F2 plants by genotype 1:2:1 according to Mendel’s law by this locus and the optimal frequency of recombination between the Xcc resistance locus and the marker (13.7%) are observed.
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About the authors
E. V. Dubina
Federal Scientific Rice Centre
Author for correspondence.
Email: lenakrug1@rambler.ru
Grand PhD in Biological Sciences, Professor of the RAS
Russian Federation, KrasnodarYu. A. Makukha
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
PhD in Biological Sciences
Russian Federation, KrasnodarS. V. Garkusha
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Corresponding member of the RAS
Russian Federation, KrasnodarO. L. Gorun
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Junior Researcher
Russian Federation, KrasnodarS. A. Lesnyak
Federal Scientific Rice Centre
Email: lenakrug1@rambler.ru
Junior Researcher
Russian Federation, KrasnodarReferences
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