Search for informative marker systems associated with loci of resistance to vascular bacteriosis in cultivated cabbage

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

This article presents the results of studies on the determination of informative DNA marker systems that provide reliable control of the presence of Xcc-loci of resistance to black rot in the breeding material of white cabbage. At the initial stage of the work, 20 molecular markers taken from the VegMarks database were tested on isogenic cabbage lines contrasting in resistance to black rot (resistant line 269-Yas12p-2 and susceptible line Pi714). It was found that only the SSR marker Ol10-C01 reveals polymorphism between contrasting samples of white cabbage. PCR analysis with the use of this polymorphic marker and phytopathological testing have been also performed on F2 plants of the hybrid combination 269-Yas12p-2 × Pi714. As a result of the statistical analysis of cleavage, it was found that the SSR marker Ol10-C01 is co-inherited with a trait of resistance to black rot, since the expected segregation of F2 plants by genotype 1:2:1 according to Mendel’s law by this locus and the optimal frequency of recombination between the Xcc resistance locus and the marker (13.7%) are observed.

全文:

受限制的访问

作者简介

E. Dubina

Federal Scientific Rice Centre

编辑信件的主要联系方式.
Email: lenakrug1@rambler.ru

Grand PhD in Biological Sciences, Professor of the RAS

俄罗斯联邦, Krasnodar

Yu. Makukha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

PhD in Biological Sciences

俄罗斯联邦, Krasnodar

S. Garkusha

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Corresponding member of the RAS

俄罗斯联邦, Krasnodar

O. Gorun

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

俄罗斯联邦, Krasnodar

S. Lesnyak

Federal Scientific Rice Centre

Email: lenakrug1@rambler.ru

Junior Researcher

俄罗斯联邦, Krasnodar

参考

  1. Batin N.V. Komp’uterniy statisticheskiy analiz dannykh. Minsk, 2008. 160 s.
  2. Koroleva S.V., Dyakunchak S.A., Sitnikov S.V. Immunologicheskaya otsenka selektsionnogo materiala pri sozdanii gibridov F1 belokochannoy kapusty s gruppovoy ustoychivost’yu k fusariozu i sosudistomu bakteriozu (metodicheskie rekomendatsii). M., 2012. 16 s.
  3. Koroleva S.V., Dyakunchak S.A., Yurchenko S.A. Development of F1 hybrids of cabbage with complex resistance in the south of Russia. Vegetable crops of Russia. 2019; 4: 16–20. https://doi.org/10.18619/2072-9146-2019-4-16-20
  4. Kutlunina N.A., Ermoshin A.A. Molekulyarno-geneticheskie metody v issledovanii rasteniy. Ekaterinburg, 2017. 142 s.
  5. Nguen M.L., Monakhos G.F., Komakhin R.A., Monakhos S.G. New resistance locus to clubroot in chromosome A05 of Chinese cabbage (Brassica rapa L.) // Rus. J. Genetics. 2018. V. 54. № 3. P. 306–315.
  6. Afrin K.S., Rahim M.A., Park J. et al. Screening of Cabbage (Brassica oleracea L.) Germplasm for Resistance to Black Rot. Mol. Biol. Rep. 2018. No. 5. PP. 773–785. https://doi.org/10.1007/s11033-018-4217-5
  7. Cruz J., Tenreiro R., Cruz L. Assessment of Diversity Xanthomonas campestris Pathovars Affecting Cruciferous Plants in Portugal and Disclosure of two novel Xcc races. Plant Pathol. 2017. No. 9. PP. 403–414 https://doi.org/10.4454/jpp.v99i2.3890
  8. Kifuji Y., Hanzaea H., Terasawa Y., Nishio T. QTL analysis of black rot resistance in cabbage using newly developed EST-SNP markers. Euphytica. 2013. No. 190. PP. 289–295. https://doi.org/10.1007/s10681-012-0847-1
  9. Lee J., Izzah N.K., Jayakodi V. Genome-wide SNP identification and QTL mapping for black rot resistance in cabbage. BMC Plant Biol. 2015. No. 15 (32). P. 11. https://doi.org/10.1186/s12870-015-0424-6
  10. Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980. V. 10. P. 4321–4325.
  11. Sharma B.B., Kalia P., Yadava D.K. et al. Genetics and molecular mapping of black rot resistance locus Xca1bc on chromosome B-7 in Ethiopian mustard. PLoS ONE. 2016. No. 11 (3). P. 13. https://doi.org/10.1371/journal.pone.0152290
  12. Singh D., Raghavendra B.T., Singh P. et al. Detection of black rot disease causing pathogen Xanthomonas campestris pv. campestris by bio-PCR from seeds and plant parts of cole crops Seed Sci. & Technol. 2014. No. 42. PP. 36–46. http://doi.org/10.15258/sst.2014.42.1.04
  13. Tonu N.N., Doullah M.A., Shimizu M. et al. Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleracea. American Journal of Plant Sciences. 2013. No. 4. PP. 11–20. http://dx.doi.org/10.4236/ajps.2013.48A002
  14. Vicente J.G., Holub E.B. Xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. Mol. Plant Pathol. 2013. No. 14 (1). PP. 2–18. https://doi.org/10.1111/j.1364-3703.2012.00833.x
  15. Vo Thi Ngok Ha, Dzhalilov F.S., Mazurin E. et al. Use of essential oils for disinfection of cabbage seed from black root Zasch. Kart. 2014. No. 2. PP. 26–28 http://www.kartofel.org/zakart/zakart2_2014.pdf

补充文件

附件文件
动作
1. JATS XML
下载
2. Figures for the article by E.V. Dubina et al. "Search for informative marker systems associated with loci of resistance to vascular bacteriosis in cabbage" (p. 15)

下载 (535KB)
索引源数据

版权所有 © Russian Academy of Sciences, 2024

##common.cookie##