Gene-Activated Bone Substitute Based on Octacalcium Phosphate and Doped with Magnesium Ions

Abstract

This work is aimed at the optimization of the phase composition, surface morphology, and microstructure of matrices based on octacalcium phosphate (OCP) in order to increase the maximum dose and dosing accuracy of immobilized nucleic acids (NAs). It is determined that the modification of the scaffold with magnesium leads to a 1.4-fold increase in binding of plasmid DNA, which can further be used for the fabrication of medical products with the specified high concentration of biologically active NAs. Preliminary in vitro evaluation shows that the addition of magnesium to OCP regulates metabolic activity of HEK-293FT cell lines.