Email this article The New Plant Expression System for the Development of Vaccines against Papillomaviruses
- Authors: Salyaev R.K.1, Rekoslavskaya N.I.1,2, Stolbikov A.S.1
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Affiliations:
- Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
- Irkutsk Scientific Center, Siberian Branch, Russian Academy of Sciences
- Issue: Vol 484, No 1 (2019)
- Pages: 52-54
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212743
- DOI: https://doi.org/10.1134/S1607672919010150
- ID: 212743
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Abstract
To enhance the synthesis of antigenic envelope proteins L1 of high-grade papillomavirus types HPV16, HPV18, HPV31, and HPV45, the sequence of the gene encoding the cucumber mosaic virus replicase (RdRP CMV) was inserted into the genetic construct. This made it possible to increase the production of these antigenic proteins to 25–27 μg/mg total soluble protein.
About the authors
R. K. Salyaev
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: salyaev@sifibr.irk.ru
Russian Federation, Irkutsk, 664033
N. I. Rekoslavskaya
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences; Irkutsk Scientific Center, Siberian Branch,Russian Academy of Sciences
Author for correspondence.
Email: rekoslavskaya@sifibr.irk.ru
Russian Federation, Irkutsk, 664033; Irkutsk, 664033
A. S. Stolbikov
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Email: rekoslavskaya@sifibr.irk.ru
Russian Federation, Irkutsk, 664033
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