Pyrazine-2-carbohydrazone of Pyridoxal 5′-Phosphate: Synthesis, Stability, Formation Kinetics, and Interaction with DNA
- Authors: Gamov G.A.1, Zavalishin M.N.1, Aleksandriyskii V.V.1, Sharnin V.A.1
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Affiliations:
- Ivanovo State University of Chemistry and Technology
- Issue: Vol 89, No 2 (2019)
- Pages: 230-235
- Section: Article
- URL: https://journals.rcsi.science/1070-3632/article/view/222744
- DOI: https://doi.org/10.1134/S1070363219020105
- ID: 222744
Cite item
Abstract
(5-Hydroxy-6-methyl-4-{(E)-[2-oxo-2-(pyrazin-2-yl)ethyl]hydrazinylidenemethyl}pyridin-3-yl)- methyl phosphate has been synthesized via the interaction of pyridoxal-5-phosphate and pyrazin-2- carbohydrazide. Its stability constants in an aqueous solution at pH = 1.9, 6.6, 7.0, and 7.4 have been determined. The kinetics of formation and hydrolysis of the hydrazone has been studied, and rate constants of the direct and reverse reactions have been calculated from the electronic absorption spectroscopy data. The interaction of the obtained hydrazone and its Cu(II) complex with calf thymus DNA has been investigated.
Keywords
About the authors
G. A. Gamov
Ivanovo State University of Chemistry and Technology
Author for correspondence.
Email: oxt705@isuct.ru
Russian Federation, Sheremetevskii pr. 7, Ivanovo, 153000
M. N. Zavalishin
Ivanovo State University of Chemistry and Technology
Email: oxt705@isuct.ru
Russian Federation, Sheremetevskii pr. 7, Ivanovo, 153000
V. V. Aleksandriyskii
Ivanovo State University of Chemistry and Technology
Email: oxt705@isuct.ru
Russian Federation, Sheremetevskii pr. 7, Ivanovo, 153000
V. A. Sharnin
Ivanovo State University of Chemistry and Technology
Email: oxt705@isuct.ru
Russian Federation, Sheremetevskii pr. 7, Ivanovo, 153000