The Hybrid Protein of the Alkaline Sensor IRR and the Fluorescent Protein GFP Retains the Functional Activity of the Receptor
- 作者: Mozhaev A.1,2, Serova O.1, Orsa A.1, Boyko A.1, Goryashchenko A.1, Deyev I.1, Petrenko A.1
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Shubnikov Institute of Crystallography, Crystallography and Photonics Research Center, Russian Academy of Sciences
- 期: 卷 45, 编号 2 (2019)
- 页面: 179-182
- 栏目: Letter to Editor
- URL: https://journals.rcsi.science/1068-1620/article/view/229187
- DOI: https://doi.org/10.1134/S1068162019020080
- ID: 229187
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详细
The insulin receptor-related receptor (IRR) is a cellular sensor of a weakly alkaline medium. Its spatial structure and the mechanism of activation have not been yet established. In the present work, a system of heterologous expression of full-length IRR tyrosine kinase has been created. A plasmid construct encoding the cDNA of the full-length human IRR fused at the C-terminus with the green fluorescent protein (GFP) has been generated, and the hybrid protein has been expressed in HEK293 line cells. It has been shown that the receptor incorporated in the hybrid protein is expressed on the cytoplasmic membrane and retains its functional activity. The resulting protein can be used in further studies of the structure and function of IRR.
作者简介
A. Mozhaev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Shubnikov Institute of Crystallography, Crystallography and Photonics Research Center, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997; Moscow, 119333
O. Serova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997
A. Orsa
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997
A. Boyko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997
A. Goryashchenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997
I. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997
A. Petrenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: petrenkoag@gmail.com
俄罗斯联邦, Moscow, 117997